Robust determination of adherent and suspended cancer cell concentration using optical density

Danni Chen, Duli Yu, Liujia Shi, Xiaoliang Guo*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Optical density is commonly used as a simple and rapid indirect measurement method to estimate biomass concentration in liquid cultures. However, the object of optical density detection is often algae cells, colonies, and other microorganisms,few studies adopt optical density to quantitatively measure the concentration of cancer cells. In this paper, different liquid media and cancer cells were used to implement a full-wavelength spectral analysis by microplate reader to find the corresponding robust wavelength. According to the experimental results, we suggest measuring cell concentration at near-infrared wavelengths, such as 850 nm, to facilitate the subsequent unification of protocols applicable to different cell types and culture conditions. Meanwhile, a portable flow cell sensor based on optical density is demonstrated. The calibration curves under various experimental conditions have high regression coefficient R2 values, all greater than 0.99, which are expected to be used for online real-time measurement of cell concentration in biological reaction processes. This study provides the feasibility of using the optical density method as a quick and easy way for indirect quantitative measurement of cancer cell concentration.

Original languageEnglish
Title of host publicationInternational Conference on Optoelectronic Materials and Devices, ICOMD 2022
EditorsQiang Huang
PublisherSPIE
ISBN (Electronic)9781510663268
DOIs
Publication statusPublished - 2023
Event2022 International Conference on Optoelectronic Materials and Devices, ICOMD 2022 - Chongqing, China
Duration: 16 Dec 202218 Dec 2022

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume12600
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Conference

Conference2022 International Conference on Optoelectronic Materials and Devices, ICOMD 2022
Country/TerritoryChina
CityChongqing
Period16/12/2218/12/22

Keywords

  • Optical density
  • biomanufacturing
  • cell concentration
  • optical sensor

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