Rapid Detection of Copper in Biological Systems Using Click Chemistry

Wenshu Zheng; Huiling Li; Wenwen Chen; Jiangjiang Zhang; Nuoxin Wang; Xuefeng Guo; Xingyu Jiang

doi:10.1002/smll.201703857

Rapid Detection of Copper in Biological Systems Using Click Chemistry

Wenshu Zheng, Huiling Li, Wenwen Chen, Jiangjiang Zhang, Nuoxin Wang, Xuefeng Guo, Xingyu Jiang^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

40 Citations (Scopus)

Abstract

A fast (1 min), straightforward but efficient, click chemistry-based system that enables the rapid detection of free copper (Cu) ions in either biological fluids or living cells without tedious pretreatment is provided. Cu can quickly induce the conjugation between graphene oxide (GO) and a fluorescent dye via click reaction. On the basis of the high specificity of bioorthogonal reaction and the effective quenching ability of GO, the assay studied in this paper can respond to Cu ions in less than 1 min with excellent selectivity and sensitivity, which is the fastest sensor for Cu as far as it is known. In addition, the application of this system is verified by performing assays in living cells and untreated urine samples from patients suffering from Wilson's Disease. Such a Cu detection system shows great promises in both fundamental research and routine clinical diagnostics.

Original language	English
Article number	1703857
Journal	Small
Volume	14
Issue number	14
DOIs	https://doi.org/10.1002/smll.201703857
Publication status	Published - 5 Apr 2018
Externally published	Yes

Keywords

Cu detection
click chemistry
graphene

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10.1002/smll.201703857

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abstract = "A fast (1 min), straightforward but efficient, click chemistry-based system that enables the rapid detection of free copper (Cu) ions in either biological fluids or living cells without tedious pretreatment is provided. Cu can quickly induce the conjugation between graphene oxide (GO) and a fluorescent dye via click reaction. On the basis of the high specificity of bioorthogonal reaction and the effective quenching ability of GO, the assay studied in this paper can respond to Cu ions in less than 1 min with excellent selectivity and sensitivity, which is the fastest sensor for Cu as far as it is known. In addition, the application of this system is verified by performing assays in living cells and untreated urine samples from patients suffering from Wilson's Disease. Such a Cu detection system shows great promises in both fundamental research and routine clinical diagnostics.",

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AU - Zheng, Wenshu

AU - Li, Huiling

AU - Chen, Wenwen

AU - Zhang, Jiangjiang

AU - Wang, Nuoxin

AU - Guo, Xuefeng

AU - Jiang, Xingyu

PY - 2018/4/5

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AB - A fast (1 min), straightforward but efficient, click chemistry-based system that enables the rapid detection of free copper (Cu) ions in either biological fluids or living cells without tedious pretreatment is provided. Cu can quickly induce the conjugation between graphene oxide (GO) and a fluorescent dye via click reaction. On the basis of the high specificity of bioorthogonal reaction and the effective quenching ability of GO, the assay studied in this paper can respond to Cu ions in less than 1 min with excellent selectivity and sensitivity, which is the fastest sensor for Cu as far as it is known. In addition, the application of this system is verified by performing assays in living cells and untreated urine samples from patients suffering from Wilson's Disease. Such a Cu detection system shows great promises in both fundamental research and routine clinical diagnostics.

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Rapid Detection of Copper in Biological Systems Using Click Chemistry

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Keywords

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