Purification and identification of soluble Catechol-O-methyltransferase from rat liver

Lina Yang*, Jianhui Yan, Yulin Deng

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Soluble catechol-O-methytransferase (S-COMT) is an important target protein due to its involvement in some human disorders. The main scope of the work was to develop an approach to purify and identify S-COMT. Its isoelectric point was S.O determined with Offgel. A simplified process including extraction, selective precipitation, ammonium sulfate precipitation, gel nitration chromatography and anion exchange chromatography was established to obtain S-COMT of a certain purity degree, The molecular was 2SkD. Mass spectrometry and mascot were employed for further identification.

Original languageEnglish
Title of host publication2010 4th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2010
DOIs
Publication statusPublished - 2010
Event4th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2010 - Chengdu, China
Duration: 18 Jun 201020 Jun 2010

Publication series

Name2010 4th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2010

Conference

Conference4th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2010
Country/TerritoryChina
CityChengdu
Period18/06/1020/06/10

Keywords

  • Identification
  • Mass spectrometry
  • Offgel isoelectric focusing
  • Purification
  • Solublecatechol-O-methyltransferase

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