Oxygen Quenching-Resistant Nanoaggregates with Aggregation-Induced Delayed Fluorescence for Time-Resolved Mapping of Intracellular Microviscosity

Fengyan Song; Xinwen Ou; Tsu Yu Chou; Junkai Liu; Hui Gao; Ruoyao Zhang; Xiaolin Huang; Zujin Zhao; Jianwei Sun; Sijie Chen; Jacky W.Y. Lam; Ben Zhong Tang

doi:10.1021/acsnano.1c11661

Oxygen Quenching-Resistant Nanoaggregates with Aggregation-Induced Delayed Fluorescence for Time-Resolved Mapping of Intracellular Microviscosity

Fengyan Song, Xinwen Ou, Tsu Yu Chou, Junkai Liu, Hui Gao^*, Ruoyao Zhang, Xiaolin Huang, Zujin Zhao, Jianwei Sun, Sijie Chen^*, Jacky W.Y. Lam, Ben Zhong Tang^*

^*Corresponding author for this work

School of Medical and Technology

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Microviscosity is a fundamental parameter in the biophysics of life science and governs numerous cellular processes. Thus, the development of real-time quantitative monitoring of microviscosity inside cells is important. The traditional probes for detecting microviscosity via time-resolved luminescence imaging (TRLI) are generally disturbed by autofluorescence or surrounding oxygen in cells. Herein, we developed loose packing nanoaggregates with aggregation-induced delayed fluorescence (FKP-POA and FKP-PTA) and free from the effect of oxygen and autofluorescence for viscosity mapping via TRLI. The feasibility of FKP-PTA nanoparticles (NPs) for microviscosity mapping through TRLI was demonstrated by monitoring the variation of microviscosity inside HepG2 cancer cells, which demonstrated a value change from 14.9 cP to 216.9 cP during the apoptosis. This indicates that FKP-PTA NP can be used as a probe for cellular microviscosity mapping to help people to understand the physiologically dynamic microenvironment. The present results are expected to promote the advancement of diagnostic and therapeutic methods to cope with related diseases.

Original language	English
Pages (from-to)	6176-6184
Number of pages	9
Journal	ACS Nano
Volume	16
Issue number	4
DOIs	https://doi.org/10.1021/acsnano.1c11661
Publication status	Published - 26 Apr 2022

Keywords

aggregation-induced delayed fluorescence
aggregation-induced emission
microviscosity probe
molecular motion
time-resolved imaging

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1021/acsnano.1c11661

Cite this

@article{2563fbc18db44793abe0119071749e93,

title = "Oxygen Quenching-Resistant Nanoaggregates with Aggregation-Induced Delayed Fluorescence for Time-Resolved Mapping of Intracellular Microviscosity",

abstract = "Microviscosity is a fundamental parameter in the biophysics of life science and governs numerous cellular processes. Thus, the development of real-time quantitative monitoring of microviscosity inside cells is important. The traditional probes for detecting microviscosity via time-resolved luminescence imaging (TRLI) are generally disturbed by autofluorescence or surrounding oxygen in cells. Herein, we developed loose packing nanoaggregates with aggregation-induced delayed fluorescence (FKP-POA and FKP-PTA) and free from the effect of oxygen and autofluorescence for viscosity mapping via TRLI. The feasibility of FKP-PTA nanoparticles (NPs) for microviscosity mapping through TRLI was demonstrated by monitoring the variation of microviscosity inside HepG2 cancer cells, which demonstrated a value change from 14.9 cP to 216.9 cP during the apoptosis. This indicates that FKP-PTA NP can be used as a probe for cellular microviscosity mapping to help people to understand the physiologically dynamic microenvironment. The present results are expected to promote the advancement of diagnostic and therapeutic methods to cope with related diseases.",

keywords = "aggregation-induced delayed fluorescence, aggregation-induced emission, microviscosity probe, molecular motion, time-resolved imaging",

author = "Fengyan Song and Xinwen Ou and Chou, {Tsu Yu} and Junkai Liu and Hui Gao and Ruoyao Zhang and Xiaolin Huang and Zujin Zhao and Jianwei Sun and Sijie Chen and Lam, {Jacky W.Y.} and Tang, {Ben Zhong}",

note = "Publisher Copyright: {\textcopyright} 2022 American Chemical Society.",

year = "2022",

month = apr,

day = "26",

doi = "10.1021/acsnano.1c11661",

language = "English",

volume = "16",

pages = "6176--6184",

journal = "ACS Nano",

issn = "1936-0851",

publisher = "American Chemical Society",

number = "4",

}

TY - JOUR

T1 - Oxygen Quenching-Resistant Nanoaggregates with Aggregation-Induced Delayed Fluorescence for Time-Resolved Mapping of Intracellular Microviscosity

AU - Song, Fengyan

AU - Ou, Xinwen

AU - Chou, Tsu Yu

AU - Liu, Junkai

AU - Gao, Hui

AU - Zhang, Ruoyao

AU - Huang, Xiaolin

AU - Zhao, Zujin

AU - Sun, Jianwei

AU - Chen, Sijie

AU - Lam, Jacky W.Y.

AU - Tang, Ben Zhong

PY - 2022/4/26

Y1 - 2022/4/26

N2 - Microviscosity is a fundamental parameter in the biophysics of life science and governs numerous cellular processes. Thus, the development of real-time quantitative monitoring of microviscosity inside cells is important. The traditional probes for detecting microviscosity via time-resolved luminescence imaging (TRLI) are generally disturbed by autofluorescence or surrounding oxygen in cells. Herein, we developed loose packing nanoaggregates with aggregation-induced delayed fluorescence (FKP-POA and FKP-PTA) and free from the effect of oxygen and autofluorescence for viscosity mapping via TRLI. The feasibility of FKP-PTA nanoparticles (NPs) for microviscosity mapping through TRLI was demonstrated by monitoring the variation of microviscosity inside HepG2 cancer cells, which demonstrated a value change from 14.9 cP to 216.9 cP during the apoptosis. This indicates that FKP-PTA NP can be used as a probe for cellular microviscosity mapping to help people to understand the physiologically dynamic microenvironment. The present results are expected to promote the advancement of diagnostic and therapeutic methods to cope with related diseases.

AB - Microviscosity is a fundamental parameter in the biophysics of life science and governs numerous cellular processes. Thus, the development of real-time quantitative monitoring of microviscosity inside cells is important. The traditional probes for detecting microviscosity via time-resolved luminescence imaging (TRLI) are generally disturbed by autofluorescence or surrounding oxygen in cells. Herein, we developed loose packing nanoaggregates with aggregation-induced delayed fluorescence (FKP-POA and FKP-PTA) and free from the effect of oxygen and autofluorescence for viscosity mapping via TRLI. The feasibility of FKP-PTA nanoparticles (NPs) for microviscosity mapping through TRLI was demonstrated by monitoring the variation of microviscosity inside HepG2 cancer cells, which demonstrated a value change from 14.9 cP to 216.9 cP during the apoptosis. This indicates that FKP-PTA NP can be used as a probe for cellular microviscosity mapping to help people to understand the physiologically dynamic microenvironment. The present results are expected to promote the advancement of diagnostic and therapeutic methods to cope with related diseases.

KW - aggregation-induced delayed fluorescence

KW - aggregation-induced emission

KW - microviscosity probe

KW - molecular motion

KW - time-resolved imaging

UR - http://www.scopus.com/inward/record.url?scp=85127672194&partnerID=8YFLogxK

U2 - 10.1021/acsnano.1c11661

DO - 10.1021/acsnano.1c11661

M3 - Article

C2 - 35318852

AN - SCOPUS:85127672194

SN - 1936-0851

VL - 16

SP - 6176

EP - 6184

JO - ACS Nano

JF - ACS Nano

IS - 4

ER -

Oxygen Quenching-Resistant Nanoaggregates with Aggregation-Induced Delayed Fluorescence for Time-Resolved Mapping of Intracellular Microviscosity

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this