Optimization of oligonucleotide pools amplification for SELEX

Cong Xiao Zhang, Xue Fei Lv, Xiang Han Wang, Hong Qing, Yu Lin Deng*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

In order to realize the amplification of oligonucleotide pools for aptamer selection, this investigation studied the Optimization of oligonucleotide amplification pools for SELEX. The PCR conditions were optimized including cycles, annealing temperature, primer concentrations and template concentrations. The study will be helpful with the aptamer selection for aptamer candidate amplification.

Original languageEnglish
Title of host publicationMedicine Sciences and Bioengineering - Proceedings of the 2014 International Conference on Medicine Sciences and Bioengineering, ICMSB 2014
EditorsMings Wang
PublisherCRC Press/Balkema
Pages749-752
Number of pages4
ISBN (Electronic)9781138026841
DOIs
Publication statusPublished - 2015
EventProceedings of the 2014 International Conference on Medicine Sciences and Bioengineering, ICMSB 2014 - Kunming, China
Duration: 16 Aug 201417 Aug 2014

Publication series

NameMedicine Sciences and Bioengineering - Proceedings of the 2014 International Conference on Medicine Sciences and Bioengineering, ICMSB 2014

Conference

ConferenceProceedings of the 2014 International Conference on Medicine Sciences and Bioengineering, ICMSB 2014
Country/TerritoryChina
CityKunming
Period16/08/1417/08/14

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