@inproceedings{17544c88846e4d55affece0baf4eac8a,
title = "Microrobotic assembly of shape-controllable microstructures to perfusable 3D cell-laden microtissues",
abstract = "Tissue engineered cellular microtissues by three-dimensional assembly to clearly model human organs in vitro is increasingly needed. In this paper, cell-encapsulated 3D microstructures with micro vessel-like lumen were fabricated using PEGDA (poly (ethylene) glycol diacrylate) hydrogels. The available DMD-based (Digital Mirror Device) photolithography system was utilized to fabricate 2D cellular micromodules in a microfluidic channel with no need of mask. The heterogeneous micromodules were assembled by a modified rail-guided microrobotic system with a straight forward strategy and high efficiency. The adjustment to align assembled micromodules to be a regular geometry relied on liquid surface tension. With rapid 2D fabrication and 3D assembly, NIH/3T3 cells encapsulated in hydrogels can be survived for over 14 days in 2D micromodules and 2days in 3D microstructures with incubation. Long-term culture of cells were performed with different concentration of PEGDA, and shown that cells viability increased by decreasing PEGDA concentration. Cell adhesion was improved by adding RGDS (Arg-Gly-Asp-Ser) peptide sequence with observation under optical microscope. The assembled 3D cellular microtissues have a significant potential to be used in regenerative medicine in the future.",
keywords = "3D assembly, PEGDA, longterm culture, microrobotic system",
author = "Juan Cui and Huaping Wang and Qing Shi and Jianing Li and Zhiqiang Zheng and Tao Sun and Qiang Huang and Toshio Fukuda",
note = "Publisher Copyright: {\textcopyright} 2017 IEEE.; 7th IEEE Annual International Conference on CYBER Technology in Automation, Control, and Intelligent Systems, CYBER 2017 ; Conference date: 31-07-2017 Through 04-08-2017",
year = "2018",
month = aug,
day = "24",
doi = "10.1109/CYBER.2017.8446611",
language = "English",
isbn = "9781538604892",
series = "2017 IEEE 7th Annual International Conference on CYBER Technology in Automation, Control, and Intelligent Systems, CYBER 2017",
publisher = "Institute of Electrical and Electronics Engineers Inc.",
pages = "13--18",
booktitle = "2017 IEEE 7th Annual International Conference on CYBER Technology in Automation, Control, and Intelligent Systems, CYBER 2017",
address = "United States",
}
