Micro-Raman spectroscopic observation of water expulsion induced destruction of hydrophobic clusters in crystalline lysozyme

Jing Jing Shou, Guang Zeng, Yun Hong Zhang*, G. Q. Lu

*Corresponding author for this work

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Abstract

In this paper, three kinds of solid lysozyme samples with different water contents were investigated by confocal Raman spectroscopy. For the rod-like lysozyme crystal with highest water content, a sudden decrease of the intensity ratio of the doublet at 1338 and 1360 cm-1 was observed when the ambient relative humidity (RH) was lower than 86%, indicating the destruction of hydrophobic clusters of lysozyme induced by the expulsion of the hydration water from the crystal. In contrast to the rod-like crystal, tetragonal crystal and floor-like precipitate with a smaller amount of water showed no change of the structures of the hydrophobic clusters when the relative humidity was decreasing. The presence of bulk water in the rod-like crystal is believed a necessary factor for the function of the hydration water which promotes the hydrophobicity of hydrophobic clusters.

Original languageEnglish
Pages (from-to)9633-9635
Number of pages3
JournalJournal of Physical Chemistry B
Volume113
Issue number29
DOIs
Publication statusPublished - 23 Jul 2009

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Shou, J. J., Zeng, G., Zhang, Y. H., & Lu, G. Q. (2009). Micro-Raman spectroscopic observation of water expulsion induced destruction of hydrophobic clusters in crystalline lysozyme. Journal of Physical Chemistry B, 113(29), 9633-9635. https://doi.org/10.1021/jp904257x