TY - GEN
T1 - Long survival observation of C. Elegans inside Environmental SEM for nanomanipulations
AU - Nakajima, Masahiro
AU - Nakanishi, Naoya
AU - Hisamoto, Naoki
AU - Takeuchi, Masaru
AU - Tajima, Hirotaka
AU - Homma, Michio
AU - Huang, Qiang
AU - Fukuda, Toshio
PY - 2013
Y1 - 2013
N2 - This paper presents a novel technique for long survival observation of Caenorhabditis elegans (C. elegans) inside Environmental-SEM (E-SEM) for nanomanipulations. We have developed the nanorobotic manipulation system inside an Environmental-Scanning Electron Microscope (E-SEM) integrated optical microscope (OM) for nanobiomanipulation platform. Our platform provides a real-time high resolution image under water-contained condition in nano-meter high-magnification image. One of the difficulties on the E-SEM observation is to maintain the biological sample on survival condition in long time. In this paper, we propose a coating method on the C. elegans or sample substrate for maintain the survival condition to keep in wet-condition. The proposed technique is useful to operate the nanomanipulation on C. elegans body, for example nano-injection of functional micro-nano beads as demonstrated in this paper.
AB - This paper presents a novel technique for long survival observation of Caenorhabditis elegans (C. elegans) inside Environmental-SEM (E-SEM) for nanomanipulations. We have developed the nanorobotic manipulation system inside an Environmental-Scanning Electron Microscope (E-SEM) integrated optical microscope (OM) for nanobiomanipulation platform. Our platform provides a real-time high resolution image under water-contained condition in nano-meter high-magnification image. One of the difficulties on the E-SEM observation is to maintain the biological sample on survival condition in long time. In this paper, we propose a coating method on the C. elegans or sample substrate for maintain the survival condition to keep in wet-condition. The proposed technique is useful to operate the nanomanipulation on C. elegans body, for example nano-injection of functional micro-nano beads as demonstrated in this paper.
UR - http://www.scopus.com/inward/record.url?scp=84894194140&partnerID=8YFLogxK
U2 - 10.1109/NANO.2013.6721021
DO - 10.1109/NANO.2013.6721021
M3 - Conference contribution
AN - SCOPUS:84894194140
SN - 9781479906758
T3 - Proceedings of the IEEE Conference on Nanotechnology
SP - 187
EP - 190
BT - 2013 13th IEEE International Conference on Nanotechnology, IEEE-NANO 2013
T2 - 2013 13th IEEE International Conference on Nanotechnology, IEEE-NANO 2013
Y2 - 5 August 2013 through 8 August 2013
ER -