TY - JOUR
T1 - Indole affects the formation of multicellular aggregate structures in Pantoea agglomerans YS19
AU - Yu, Xuemei
AU - Jiang, Jing
AU - Liang, Chen
AU - Zhang, Xiao
AU - Wang, Jieru
AU - Shen, Delong
AU - Feng, Yongjun
N1 - Publisher Copyright:
© 2016 Applied Microbiology, Molecular and Cellular Biosciences Research Foundation.
PY - 2016/2/29
Y1 - 2016/2/29
N2 - Pantoea agglomerans YS19 is an endophytic diazotrophic bacterium isolated from rice. As well as having the ability to form a biofilm, as do most bacteria, it is characterized by the formation of a unique multicellular aggregate structure called symplasmata. Indole is traditionally known as a metabolite of the amino acid tryptophan, which, however, has recently been shown to participate in various regulations of bacterial physiological processes, including stress resistance, quorum sensing and biofilm formation. Here, an indole signal was found to promote symplasmata formation, yet inhibit biofilm formation, indicating different regulatory pathways of indole in the construction of the two structures. However, symplasmata showed almost an equivalent stress-resistant capability, as compared with biofilms, for YS19 to confront acids, heavy metals (Cu2+), and UV treatments. Moreover, indole was tested to show a promoting effect on exopolysaccharides (EPS) production and an inhibition effect on the expression of an outer membrane protein OmpW. These results provide evidence for understanding the regulatory mechanisms of indole on such multicellular aggregates.
AB - Pantoea agglomerans YS19 is an endophytic diazotrophic bacterium isolated from rice. As well as having the ability to form a biofilm, as do most bacteria, it is characterized by the formation of a unique multicellular aggregate structure called symplasmata. Indole is traditionally known as a metabolite of the amino acid tryptophan, which, however, has recently been shown to participate in various regulations of bacterial physiological processes, including stress resistance, quorum sensing and biofilm formation. Here, an indole signal was found to promote symplasmata formation, yet inhibit biofilm formation, indicating different regulatory pathways of indole in the construction of the two structures. However, symplasmata showed almost an equivalent stress-resistant capability, as compared with biofilms, for YS19 to confront acids, heavy metals (Cu2+), and UV treatments. Moreover, indole was tested to show a promoting effect on exopolysaccharides (EPS) production and an inhibition effect on the expression of an outer membrane protein OmpW. These results provide evidence for understanding the regulatory mechanisms of indole on such multicellular aggregates.
KW - Biofilm
KW - Indole
KW - Pantoea agglomerans
KW - Symplasmata
UR - http://www.scopus.com/inward/record.url?scp=84959346730&partnerID=8YFLogxK
U2 - 10.2323/jgam.62.31
DO - 10.2323/jgam.62.31
M3 - Article
C2 - 26923129
AN - SCOPUS:84959346730
SN - 0022-1260
VL - 62
SP - 31
EP - 37
JO - Journal of General and Applied Microbiology
JF - Journal of General and Applied Microbiology
IS - 1
ER -