High ohmic resistor hyphenated gel loading tip nano-electrospray ionization source for mini mass spectrometer

Md Matiur Rahman*, Debo Wu, Konstantin Chingin, Wei Xu, Huanwen Chen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

The deployment of mini mass spectrometers on the field strongly demands efficient ionization sources that are easy-to-operate. Nano-electrospray (nESI) ion source has been widely used in the field of chemistry, biology, medicine, pharmaceutical industry, clinical assessment and forensic science. In this study, a high ohmic resistor hyphenated gel loading tip nESI source was coupled with our home developed mini mass spectrometer. This ionization source has the advantages of simple-in-design, disposable and low-in-cost, therefore it could be frequently used for analysis of aqueous samples without leading to cross contamination. Performances of the gel loading tip nESI emitter were similar to pulled glass capillary, and highly compatible for the analysis of biomolecule in aqueous solution. Different peptide and small molecules have been confirmed with a continuous atmospheric pressure-interfaced (CAPI) mini mass spectrometer. The corona discharge, which was usually observed at nESI emitter tip under high aqueous solvent conditions, resulting in low ion intensity, has been successfully quenched using a 10 GΩ resistor in both a pulled glass capillary and a gel loading tip as nESI emitter in this study. Compared with conventional ESI, the metal wire assisted gel loading tip facilitated loading and direct analysis of biological samples without sample pretreatment.

Original languageEnglish
Pages (from-to)59-66
Number of pages8
JournalTalanta
Volume202
DOIs
Publication statusPublished - 1 Sept 2019

Fingerprint

Dive into the research topics of 'High ohmic resistor hyphenated gel loading tip nano-electrospray ionization source for mini mass spectrometer'. Together they form a unique fingerprint.

Cite this