TY - JOUR
T1 - Europium-Doped Nanoparticles for Cellular Luminescence Lifetime Imaging via Multiple Manipulations of Aggregation State
AU - Fang, Fang
AU - Zhao, Dongxu
AU - Zhang, Yinfeng
AU - Li, Min
AU - Ye, Jun
AU - Zhang, Jinfeng
N1 - Publisher Copyright:
© 2020 American Chemical Society.
PY - 2020/8/17
Y1 - 2020/8/17
N2 - Luminescence lifetime imaging (LLIM) holds great promise in biomedical research owing to its excellent sensitivity and remarkable temporal-spatial resolution, but the development of effective long-lived luminescent probes for LLIM remains an unmet need. Herein, we designed two water-dispersible europium-doped nanoparticles (Eu-D1 and Eu-D2 NPs) for cellular LLIM, showing intense red luminescence emission and an increased lifetime of 4 orders of magnitude (56 000 times) than undoped NPs, which could be attributed to the multiple manipulations of their aggregation states, including efficient Förster resonance energy transfer (FRET), suppressed self-quenching, and restrained solvent effects. With good bioavailability, the as-developed NPs were demonstrated as reliable candidates for long-lifetime imaging agents. Such a simple and versatile strategy could be extended to develop various luminescent nanoprobes for advanced high-precision bioimaging.
AB - Luminescence lifetime imaging (LLIM) holds great promise in biomedical research owing to its excellent sensitivity and remarkable temporal-spatial resolution, but the development of effective long-lived luminescent probes for LLIM remains an unmet need. Herein, we designed two water-dispersible europium-doped nanoparticles (Eu-D1 and Eu-D2 NPs) for cellular LLIM, showing intense red luminescence emission and an increased lifetime of 4 orders of magnitude (56 000 times) than undoped NPs, which could be attributed to the multiple manipulations of their aggregation states, including efficient Förster resonance energy transfer (FRET), suppressed self-quenching, and restrained solvent effects. With good bioavailability, the as-developed NPs were demonstrated as reliable candidates for long-lifetime imaging agents. Such a simple and versatile strategy could be extended to develop various luminescent nanoprobes for advanced high-precision bioimaging.
KW - FRET effect
KW - aggregation-caused quenching effect
KW - cellular luminescence lifetime imaging
KW - europium complex
KW - nanoprobes
UR - http://www.scopus.com/inward/record.url?scp=85090941109&partnerID=8YFLogxK
U2 - 10.1021/acsabm.0c00580
DO - 10.1021/acsabm.0c00580
M3 - Article
AN - SCOPUS:85090941109
SN - 2576-6422
VL - 3
SP - 5103
EP - 5110
JO - ACS Applied Bio Materials
JF - ACS Applied Bio Materials
IS - 8
ER -