Abstract
A sensitive method based on the fluorescence quenching effect of the Tb3+-Tiron complex is proposed for the determination of alkali-labile phosphoprotein phosphorus (ALP) released from fish plasma. The detection limit was 5.4 ng/ml (S/N=2), and the relative standard deviation of the quenching effect (6 replicates) was 4.6%. The results obtained by the proposed method were in good agreement with those obtained by the colorimetric assay. The advantages of the present method are its relatively simple detection procedure, the lack of toxic organic solvents, and high sensitivity.
Original language | English |
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Pages (from-to) | 616-621 |
Number of pages | 6 |
Journal | Journal of Environmental Sciences (China) |
Volume | 19 |
Issue number | 5 |
DOIs | |
Publication status | Published - 2007 |
Externally published | Yes |
Keywords
- Chinese loach
- Tb-Tiron complex
- alkali-labile phosphoprotein phosphorus (ALP)
- fluorescence quenching
- vitellogenin
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LV, X. F., ZHAO, Y. B., ZHOU, Q. F., JIANG, G. B., & SONG, M. Y. (2007). Determination of alkali-labile phosphoprotein phosphorus from fish plasma using the Tb3+-tiron complex as a fluorescence probe. Journal of Environmental Sciences (China), 19(5), 616-621. https://doi.org/10.1016/S1001-0742(07)60102-8