Detection of 4 Relative Substances in Clarithromycin by HPLC

Li Quan Sun; Meng Nan Zhang; Bu Fang Ma; Guo Wei Yao; Ai Qin Luo

doi:10.15918/j.tbit1001-0645.2017.09.019

Detection of 4 Relative Substances in Clarithromycin by HPLC

Li Quan Sun, Meng Nan Zhang, Bu Fang Ma, Guo Wei Yao, Ai Qin Luo

School of Life Science

Beijing Institute of Technology

Research output: Contribution to journal › Article › peer-review

Abstract

An effective method was established and verified for the detection of clarithromycin and its relative substances J, M, N and K by HPLC. Clarithromycin relative substances sample was separated by isocratic elution with a Purospher STAR LP RP-18e C18 column and with the mobile phase 0.067 mol/L KH₂PO₄ solution (pH 4.0)/acetonitrile=60/40, the flow rate of 1.2 mL/min, the column temperature 35℃ and detection wavelength 205 nm, and the separation degrees were greater than 1.2. Analysis results show that, the method can provide good linearity (R>0.999) for all relative substances at the concentration range of 20~240 μg/mL, the average recovery rates keep at 87.80%~121.17%, its operation is simple and efficient, and can be able to simultaneously detect J, M, N and K in clarithromycin.

Original language	English
Pages (from-to)	986-990
Number of pages	5
Journal	Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology
Volume	37
Issue number	9
DOIs	https://doi.org/10.15918/j.tbit1001-0645.2017.09.019
Publication status	Published - 1 Sept 2017

Keywords

Clarithromycin
Detection
HPLC
Relative substances

Access to Document

10.15918/j.tbit1001-0645.2017.09.019

Cite this

@article{3f76f05475be4e21a2bf9cd063389a67,

title = "Detection of 4 Relative Substances in Clarithromycin by HPLC",

abstract = "An effective method was established and verified for the detection of clarithromycin and its relative substances J, M, N and K by HPLC. Clarithromycin relative substances sample was separated by isocratic elution with a Purospher STAR LP RP-18e C18 column and with the mobile phase 0.067 mol/L KH2PO4 solution (pH 4.0)/acetonitrile=60/40, the flow rate of 1.2 mL/min, the column temperature 35℃ and detection wavelength 205 nm, and the separation degrees were greater than 1.2. Analysis results show that, the method can provide good linearity (R>0.999) for all relative substances at the concentration range of 20~240 μg/mL, the average recovery rates keep at 87.80%~121.17%, its operation is simple and efficient, and can be able to simultaneously detect J, M, N and K in clarithromycin.",

keywords = "Clarithromycin, Detection, HPLC, Relative substances",

author = "Sun, {Li Quan} and Zhang, {Meng Nan} and Ma, {Bu Fang} and Yao, {Guo Wei} and Luo, {Ai Qin}",

year = "2017",

month = sep,

day = "1",

doi = "10.15918/j.tbit1001-0645.2017.09.019",

language = "English",

volume = "37",

pages = "986--990",

journal = "Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology",

issn = "1001-0645",

publisher = "Beijing Institute of Technology",

number = "9",

}

TY - JOUR

T1 - Detection of 4 Relative Substances in Clarithromycin by HPLC

AU - Sun, Li Quan

AU - Zhang, Meng Nan

AU - Ma, Bu Fang

AU - Yao, Guo Wei

AU - Luo, Ai Qin

PY - 2017/9/1

Y1 - 2017/9/1

N2 - An effective method was established and verified for the detection of clarithromycin and its relative substances J, M, N and K by HPLC. Clarithromycin relative substances sample was separated by isocratic elution with a Purospher STAR LP RP-18e C18 column and with the mobile phase 0.067 mol/L KH2PO4 solution (pH 4.0)/acetonitrile=60/40, the flow rate of 1.2 mL/min, the column temperature 35℃ and detection wavelength 205 nm, and the separation degrees were greater than 1.2. Analysis results show that, the method can provide good linearity (R>0.999) for all relative substances at the concentration range of 20~240 μg/mL, the average recovery rates keep at 87.80%~121.17%, its operation is simple and efficient, and can be able to simultaneously detect J, M, N and K in clarithromycin.

AB - An effective method was established and verified for the detection of clarithromycin and its relative substances J, M, N and K by HPLC. Clarithromycin relative substances sample was separated by isocratic elution with a Purospher STAR LP RP-18e C18 column and with the mobile phase 0.067 mol/L KH2PO4 solution (pH 4.0)/acetonitrile=60/40, the flow rate of 1.2 mL/min, the column temperature 35℃ and detection wavelength 205 nm, and the separation degrees were greater than 1.2. Analysis results show that, the method can provide good linearity (R>0.999) for all relative substances at the concentration range of 20~240 μg/mL, the average recovery rates keep at 87.80%~121.17%, its operation is simple and efficient, and can be able to simultaneously detect J, M, N and K in clarithromycin.

KW - Clarithromycin

KW - Detection

KW - HPLC

KW - Relative substances

UR - http://www.scopus.com/inward/record.url?scp=85032469999&partnerID=8YFLogxK

U2 - 10.15918/j.tbit1001-0645.2017.09.019

DO - 10.15918/j.tbit1001-0645.2017.09.019

M3 - Article

AN - SCOPUS:85032469999

SN - 1001-0645

VL - 37

SP - 986

EP - 990

JO - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology

JF - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology

IS - 9

ER -

Detection of 4 Relative Substances in Clarithromycin by HPLC

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this