Chitinophaga solisilvae sp. Nov., isolated from forest soil

Weiwei Ping, Ying Zhang, Huancheng Pang, Jun Zhang, Dai Li, Yuyi Li, Jianli Zhang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

A Gram-stain-negative, strictly aerobic, non-motile and rod-shaped bacterial strain, O9T, was isolated from a forest soil sample collected at Dai, Xishuangbanna, Yunnan Province, PR China. Strain O9T grew optimally at pH 7.0, at 28‒30 °C and in the absence of NaCl. 16S rRNA gene sequence analysis placed strain O9T within the genus Chitinophaga of the family Chitinophagaceae, with Chitinophaga terrae KP01T (97.8%), Chitinophaga jiangningensis JN53T (97.7%), Chitinophaga niastensis JS16-4T (97.4%), Chitin-ophaga qingshengii JN246T (97.3%) and Chitinophaga dinghuensis DHOC24T (97.3%) as its closest relatives. Strain O9T hydrolysed casein, gelatin and Tween 80. It could not assimilate l-arabinose, l-rhamnose, sucrose, melibiose, gentiobiose or d-fructose as a carbon source. It was negative for esterase lipase (C8) and β-glucosidase. Phosphatidylethanolamine was the predominant polar lipid. The major respiratory quinone of strain O9T was MK-7. Its major fatty acids were iso-C15:0 (34.2%), C16:1 ω5c (20.9%) and iso-C17:0 3-OH (12.6%). The genomic DNA G+C content of strain O9T was 49.0 mol% based on total genome calculations. The average nucleotide identity score between the genomic sequence of strain O9T and that of Chitinophaga terrae KP01T was 72.9%. The Genome-to-Genome Distance Calculator showed that DNA‒DNA hybridization values for strain O9T and Chitinophaga terrae KP01T were 13.6, 21.1 and 14.4%, respectively. Based on the polyphasic taxonomic data, strain O9T represents a novel species of the genus Chitinophaga, for which the name Chitinophaga solisilvae sp. nov. is proposed. The type strain is O9T (=CGMCC 1.12462T=KCTC 32404T).

Original languageEnglish
Article number004350
Pages (from-to)4808-4815
Number of pages8
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume70
Issue number8
DOIs
Publication statusPublished - 2020

Keywords

  • Chitinophaga
  • New species
  • Polyphasic taxonomy

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