Cell Culture of Nerve Cell and Immune Cell in CO₂-Free Environment Based on Microfludic Chip

Space station is important to space biology and it is difficult to provide CO₂ environment, a crucial element to cell culture. Meanwhile, a controllable and automatic method of cell culture is needed to perform the research in space station. Consequently, the establishment of cell culture based on microfluidic chip in a CO₂-free environment is extremely important. Firstly, changed the component of medium (Initial pH of the medium was changed, then adding up exogenous CO₂ into the medium, finally adding up buffer in culture medium), and performed the cell culture in CO₂-free environment (the co-culture of U87 MG and SHSY-5Y and THP-1), then used MTS method to determine the cell viability. Secondly, after adding different proportions of HEPES, performed the cell culture in CO₂-free environment, the cell viability was determinated by MTS method. Finally, according to the optimal proportion of HEPES, the cells were cultured in microfluidic chip. It was concluded that in CO₂-free environment, cells in microfluidic chip would survive in the medium with 3% HEPES for 5 days.