Binding mechanism of meso-tetrakis (4-(N-methylpyridiumyl)) porphyrin TMPyP4 with the single-strand DNA 5'-TTAGGG-3'

Hui Juan Zhang; Xu Kun Chen; Si Ping Pang

Binding mechanism of meso-tetrakis (4-(N-methylpyridiumyl)) porphyrin TMPyP4 with the single-strand DNA 5'-TTAGGG-3'

Hui Juan Zhang^*, Xu Kun Chen, Si Ping Pang

^*Corresponding author for this work

School of Material Science and Engineering

Beijing Institute of Technology

Research output: Contribution to journal › Article › peer-review

Abstract

The binding mechanism of meso-tetrakis (4-(N-methylpyridiumyl)) porphyrin (TMPyP4) and the telomeric sequence DNA 5'-TTAGGG-3' (S6) has been explored by means of circular dichroism spectrum, steady-state absorption, steady-state fluorescence and picosecond time-resolved fluorescence spectrums. DNA adopts the single-strand conformation in Tris-HCl buffer without any metal ions. The binding constant and the saturated binding number between TMPyP4 and S6 DNA were determined as 1.51 × 10⁶ (mol/L)^-1 and 1.2, respectively, according to steady-state absorption spectroscopy. Furthermore, on the findings of time-resolved fluorescence spectroscopic technique, the binding modes can be presumed.

Original language	English
Pages (from-to)	1365-1368+1373
Journal	Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology
Volume	30
Issue number	11
Publication status	Published - Nov 2010

Keywords

Binding mechanism
Single-strand DNA
Spectroscopic technique
TMPyP4

Cite this

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title = "Binding mechanism of meso-tetrakis (4-(N-methylpyridiumyl)) porphyrin TMPyP4 with the single-strand DNA 5'-TTAGGG-3'",

abstract = "The binding mechanism of meso-tetrakis (4-(N-methylpyridiumyl)) porphyrin (TMPyP4) and the telomeric sequence DNA 5'-TTAGGG-3' (S6) has been explored by means of circular dichroism spectrum, steady-state absorption, steady-state fluorescence and picosecond time-resolved fluorescence spectrums. DNA adopts the single-strand conformation in Tris-HCl buffer without any metal ions. The binding constant and the saturated binding number between TMPyP4 and S6 DNA were determined as 1.51 × 106 (mol/L)-1 and 1.2, respectively, according to steady-state absorption spectroscopy. Furthermore, on the findings of time-resolved fluorescence spectroscopic technique, the binding modes can be presumed.",

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AU - Chen, Xu Kun

AU - Pang, Si Ping

PY - 2010/11

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N2 - The binding mechanism of meso-tetrakis (4-(N-methylpyridiumyl)) porphyrin (TMPyP4) and the telomeric sequence DNA 5'-TTAGGG-3' (S6) has been explored by means of circular dichroism spectrum, steady-state absorption, steady-state fluorescence and picosecond time-resolved fluorescence spectrums. DNA adopts the single-strand conformation in Tris-HCl buffer without any metal ions. The binding constant and the saturated binding number between TMPyP4 and S6 DNA were determined as 1.51 × 106 (mol/L)-1 and 1.2, respectively, according to steady-state absorption spectroscopy. Furthermore, on the findings of time-resolved fluorescence spectroscopic technique, the binding modes can be presumed.

AB - The binding mechanism of meso-tetrakis (4-(N-methylpyridiumyl)) porphyrin (TMPyP4) and the telomeric sequence DNA 5'-TTAGGG-3' (S6) has been explored by means of circular dichroism spectrum, steady-state absorption, steady-state fluorescence and picosecond time-resolved fluorescence spectrums. DNA adopts the single-strand conformation in Tris-HCl buffer without any metal ions. The binding constant and the saturated binding number between TMPyP4 and S6 DNA were determined as 1.51 × 106 (mol/L)-1 and 1.2, respectively, according to steady-state absorption spectroscopy. Furthermore, on the findings of time-resolved fluorescence spectroscopic technique, the binding modes can be presumed.

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KW - Spectroscopic technique

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Binding mechanism of meso-tetrakis (4-(N-methylpyridiumyl)) porphyrin TMPyP4 with the single-strand DNA 5'-TTAGGG-3'

Abstract

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