Wang, W., Li, M., Wei, Z., Wang, Z., Bu, X., Lai, W., Yang, S., Gong, H., Zheng, H., Wang, Y., Liu, Y., Li, Q., Fang, Q., & Hu, Z. (2014). Bimodal imprint chips for peptide screening: Integration of high-throughput sequencing by MS and affinity analyses by surface plasmon resonance imaging. Analytical Chemistry, 86(8), 3703-3707. https://doi.org/10.1021/ac500465e
Wang, Weizhi ; Li, Menglin ; Wei, Zewen et al. / Bimodal imprint chips for peptide screening : Integration of high-throughput sequencing by MS and affinity analyses by surface plasmon resonance imaging. In: Analytical Chemistry. 2014 ; Vol. 86, No. 8. pp. 3703-3707.
@article{64d5c09bba3e4d89ad68c7df69c0ff20,
title = "Bimodal imprint chips for peptide screening: Integration of high-throughput sequencing by MS and affinity analyses by surface plasmon resonance imaging",
abstract = "Peptide probes and drugs have widespread applications in disease diagnostics and therapy. The demand for peptides ligands with high affinity and high specificity toward various targets has surged in the biomedical field in recent years. The traditional peptide screening procedure involves selection, sequencing, and characterization steps, and each step is manual and tedious. Herein, we developed a bimodal imprint microarray system to embrace the whole peptide screening process. Silver-sputtered silicon chip fabricated with microwell array can trap and pattern the candidate peptide beads in a one-well-one-bead manner. Peptides on beads were photocleaved in situ. A portion of the peptide in each well was transferred to a gold-coated chip to print the peptide array for high-throughput affinity analyses by surface plasmon resonance imaging (SPRi), and the peptide left in the silver-sputtered chip was ready for in situ single bead sequencing by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Using the bimodal imprint chip system, affinity peptides toward AHA were efficiently screened out from the 7 × 104 peptide library. The method provides a solution for high efficiency peptide screening.",
author = "Weizhi Wang and Menglin Li and Zewen Wei and Zihua Wang and Xiangli Bu and Wenjia Lai and Shu Yang and He Gong and Hui Zheng and Yuqiao Wang and Ying Liu and Qin Li and Qiaojun Fang and Zhiyuan Hu",
year = "2014",
month = apr,
day = "15",
doi = "10.1021/ac500465e",
language = "English",
volume = "86",
pages = "3703--3707",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "8",
}
Wang, W, Li, M, Wei, Z, Wang, Z, Bu, X, Lai, W, Yang, S, Gong, H, Zheng, H, Wang, Y, Liu, Y, Li, Q, Fang, Q & Hu, Z 2014, 'Bimodal imprint chips for peptide screening: Integration of high-throughput sequencing by MS and affinity analyses by surface plasmon resonance imaging', Analytical Chemistry, vol. 86, no. 8, pp. 3703-3707. https://doi.org/10.1021/ac500465e
Bimodal imprint chips for peptide screening: Integration of high-throughput sequencing by MS and affinity analyses by surface plasmon resonance imaging. /
Wang, Weizhi; Li, Menglin
; Wei, Zewen et al.
In:
Analytical Chemistry, Vol. 86, No. 8, 15.04.2014, p. 3703-3707.
Research output: Contribution to journal › Article › peer-review
TY - JOUR
T1 - Bimodal imprint chips for peptide screening
T2 - Integration of high-throughput sequencing by MS and affinity analyses by surface plasmon resonance imaging
AU - Wang, Weizhi
AU - Li, Menglin
AU - Wei, Zewen
AU - Wang, Zihua
AU - Bu, Xiangli
AU - Lai, Wenjia
AU - Yang, Shu
AU - Gong, He
AU - Zheng, Hui
AU - Wang, Yuqiao
AU - Liu, Ying
AU - Li, Qin
AU - Fang, Qiaojun
AU - Hu, Zhiyuan
PY - 2014/4/15
Y1 - 2014/4/15
N2 - Peptide probes and drugs have widespread applications in disease diagnostics and therapy. The demand for peptides ligands with high affinity and high specificity toward various targets has surged in the biomedical field in recent years. The traditional peptide screening procedure involves selection, sequencing, and characterization steps, and each step is manual and tedious. Herein, we developed a bimodal imprint microarray system to embrace the whole peptide screening process. Silver-sputtered silicon chip fabricated with microwell array can trap and pattern the candidate peptide beads in a one-well-one-bead manner. Peptides on beads were photocleaved in situ. A portion of the peptide in each well was transferred to a gold-coated chip to print the peptide array for high-throughput affinity analyses by surface plasmon resonance imaging (SPRi), and the peptide left in the silver-sputtered chip was ready for in situ single bead sequencing by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Using the bimodal imprint chip system, affinity peptides toward AHA were efficiently screened out from the 7 × 104 peptide library. The method provides a solution for high efficiency peptide screening.
AB - Peptide probes and drugs have widespread applications in disease diagnostics and therapy. The demand for peptides ligands with high affinity and high specificity toward various targets has surged in the biomedical field in recent years. The traditional peptide screening procedure involves selection, sequencing, and characterization steps, and each step is manual and tedious. Herein, we developed a bimodal imprint microarray system to embrace the whole peptide screening process. Silver-sputtered silicon chip fabricated with microwell array can trap and pattern the candidate peptide beads in a one-well-one-bead manner. Peptides on beads were photocleaved in situ. A portion of the peptide in each well was transferred to a gold-coated chip to print the peptide array for high-throughput affinity analyses by surface plasmon resonance imaging (SPRi), and the peptide left in the silver-sputtered chip was ready for in situ single bead sequencing by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Using the bimodal imprint chip system, affinity peptides toward AHA were efficiently screened out from the 7 × 104 peptide library. The method provides a solution for high efficiency peptide screening.
UR - http://www.scopus.com/inward/record.url?scp=84898892414&partnerID=8YFLogxK
U2 - 10.1021/ac500465e
DO - 10.1021/ac500465e
M3 - Article
C2 - 24641070
AN - SCOPUS:84898892414
SN - 0003-2700
VL - 86
SP - 3703
EP - 3707
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 8
ER -
Wang W, Li M, Wei Z, Wang Z, Bu X, Lai W et al. Bimodal imprint chips for peptide screening: Integration of high-throughput sequencing by MS and affinity analyses by surface plasmon resonance imaging. Analytical Chemistry. 2014 Apr 15;86(8):3703-3707. doi: 10.1021/ac500465e