Automatic single cell transfer module

Akiyuki Hasegawa; Huseyin Uvet; Kenichi Ohara; Tomohito Takubo; Yasushi Mae; Tatsuo Arai

doi:10.1109/ROBIO.2009.5420614

Automatic single cell transfer module

Akiyuki Hasegawa^*, Huseyin Uvet, Kenichi Ohara, Tomohito Takubo, Yasushi Mae, Tatsuo Arai

^*Corresponding author for this work

Osaka University

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

In this paper, we presented a design for an automated single cell loading and supply system that can be used with complex microfluidic applications requiring single cell loading such as the current nuclear transplantation method. The aim of the system is to automatically transfer mammalian donor (∼15μm) or egg (∼100μm) cells one by one from a container to a PDMS micro-channel and then transport them to other modules. The system consists of two main parts; a single cell suction module, and a PDMS-based microfluidic chip controlled by an external pump. The desired number of vacuumed cells can be directed into the microfluidic chip and stored in a docking area. From the batch, they can be moved to next module by activating pneumatic pressure valves located on two sides of the chip. The entire mechanism is combined with monitoring systems that perform the detection/tracking and control program.

Original language	English
Title of host publication	2009 IEEE International Conference on Robotics and Biomimetics, ROBIO 2009
Pages	147-152
Number of pages	6
DOIs	https://doi.org/10.1109/ROBIO.2009.5420614
Publication status	Published - 2009
Externally published	Yes
Event	2009 IEEE International Conference on Robotics and Biomimetics, ROBIO 2009 - Guilin, China Duration: 19 Dec 2009 → 23 Dec 2009

Publication series

Name	2009 IEEE International Conference on Robotics and Biomimetics, ROBIO 2009

Conference

Conference	2009 IEEE International Conference on Robotics and Biomimetics, ROBIO 2009
Country/Territory	China
City	Guilin
Period	19/12/09 → 23/12/09

Keywords

Micro-nano robotics
Microfluidic chips
Single cell applications
Vision systems

Access to Document

10.1109/ROBIO.2009.5420614

Cite this

@inproceedings{1ab7e2e290e44fd2a8240b9414124e96,

title = "Automatic single cell transfer module",

abstract = "In this paper, we presented a design for an automated single cell loading and supply system that can be used with complex microfluidic applications requiring single cell loading such as the current nuclear transplantation method. The aim of the system is to automatically transfer mammalian donor (∼15μm) or egg (∼100μm) cells one by one from a container to a PDMS micro-channel and then transport them to other modules. The system consists of two main parts; a single cell suction module, and a PDMS-based microfluidic chip controlled by an external pump. The desired number of vacuumed cells can be directed into the microfluidic chip and stored in a docking area. From the batch, they can be moved to next module by activating pneumatic pressure valves located on two sides of the chip. The entire mechanism is combined with monitoring systems that perform the detection/tracking and control program.",

keywords = "Micro-nano robotics, Microfluidic chips, Single cell applications, Vision systems",

author = "Akiyuki Hasegawa and Huseyin Uvet and Kenichi Ohara and Tomohito Takubo and Yasushi Mae and Tatsuo Arai",

year = "2009",

doi = "10.1109/ROBIO.2009.5420614",

language = "English",

isbn = "9781424447756",

series = "2009 IEEE International Conference on Robotics and Biomimetics, ROBIO 2009",

pages = "147--152",

booktitle = "2009 IEEE International Conference on Robotics and Biomimetics, ROBIO 2009",

note = "2009 IEEE International Conference on Robotics and Biomimetics, ROBIO 2009 ; Conference date: 19-12-2009 Through 23-12-2009",

}

Hasegawa, A, Uvet, H, Ohara, K, Takubo, T, Mae, Y & Arai, T 2009, Automatic single cell transfer module. in 2009 IEEE International Conference on Robotics and Biomimetics, ROBIO 2009., 5420614, 2009 IEEE International Conference on Robotics and Biomimetics, ROBIO 2009, pp. 147-152, 2009 IEEE International Conference on Robotics and Biomimetics, ROBIO 2009, Guilin, China, 19/12/09. https://doi.org/10.1109/ROBIO.2009.5420614

Automatic single cell transfer module. / Hasegawa, Akiyuki; Uvet, Huseyin; Ohara, Kenichi et al.
2009 IEEE International Conference on Robotics and Biomimetics, ROBIO 2009. 2009. p. 147-152 5420614 (2009 IEEE International Conference on Robotics and Biomimetics, ROBIO 2009).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

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AU - Uvet, Huseyin

AU - Ohara, Kenichi

AU - Takubo, Tomohito

AU - Mae, Yasushi

AU - Arai, Tatsuo

PY - 2009

Y1 - 2009

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AB - In this paper, we presented a design for an automated single cell loading and supply system that can be used with complex microfluidic applications requiring single cell loading such as the current nuclear transplantation method. The aim of the system is to automatically transfer mammalian donor (∼15μm) or egg (∼100μm) cells one by one from a container to a PDMS micro-channel and then transport them to other modules. The system consists of two main parts; a single cell suction module, and a PDMS-based microfluidic chip controlled by an external pump. The desired number of vacuumed cells can be directed into the microfluidic chip and stored in a docking area. From the batch, they can be moved to next module by activating pneumatic pressure valves located on two sides of the chip. The entire mechanism is combined with monitoring systems that perform the detection/tracking and control program.

KW - Micro-nano robotics

KW - Microfluidic chips

KW - Single cell applications

KW - Vision systems

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T3 - 2009 IEEE International Conference on Robotics and Biomimetics, ROBIO 2009

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BT - 2009 IEEE International Conference on Robotics and Biomimetics, ROBIO 2009

T2 - 2009 IEEE International Conference on Robotics and Biomimetics, ROBIO 2009

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ER -

Automatic single cell transfer module

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