An ESIPT-based fluorescent probe with large Stokes shift for peroxynitrite detection in HeLa cells and zebrafish

Jin Yu, Wei Shu*, Hao Kang, Rubing Han, Xiaoli Zhang, Rubo Zhang, Jing Jing*, Xiaoling Zhang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

The quick coupled reaction of nitric oxide (NO) with superoxide free radicals (·O2) produces peroxynitrite (ONOO), it is a powerful biological oxidant. And according to interact with proteins, lipids, nucleic acids and other organisms, the ONOO eventually leads to all sorts of diseases, for instance, Alzheimer's disease, diabetes, cancer, inflammation. To fully understand the multiple pathological processes of ONOO in vivo, it is imperative to exploit effective tools to realize the precise, fast and sensitive detection of endogenous ONOO. We described a novel fluorescent probe named YV in this work, covering the synthesis, characterization and biological application. YV can detect ONOO by means of fluorescence imaging. Because of the borate group cleavage, YV exhibited a fluorescence intensity change toward ONOO. It was, the principle of ESIPT that resulted in fluorescence enhancement. The probe YV shows a series of advantages such as high selectivity, fast response, significant Stokes shift and low detection limit. Fluorescence imaging can be used to identify variations in ONOO in Hela cells and zebrafish. YV will be a robust imaging tool for detecting endogenous ONOO.

Original languageEnglish
Article number110334
JournalDyes and Pigments
Volume204
DOIs
Publication statusPublished - Aug 2022

Keywords

  • Bioimaging
  • ESIPT
  • Fluorescent probe
  • Peroxynitrite
  • Zebrafish

Fingerprint

Dive into the research topics of 'An ESIPT-based fluorescent probe with large Stokes shift for peroxynitrite detection in HeLa cells and zebrafish'. Together they form a unique fingerprint.

Cite this