Jia, W., Lu, Z., Fu, Y., Wang, H. P., Wang, L. H., Chi, H., Yuan, Z. F., Zheng, Z. B., Song, L. N., Han, H. H., Liang, Y. M., Wang, J. L., Cai, Y., Zhang, Y. K., Deng, Y. L., Ying, W. T., He, S. M., & Qian, X. H. (2009). A strategy for precise and large scale identification of core fucosylated glycoproteins. Molecular and Cellular Proteomics, 8(5), 913-923. https://doi.org/10.1074/mcp.M800504-MCP200
Jia, Wei ; Lu, Zhuang ; Fu, Yan et al. / A strategy for precise and large scale identification of core fucosylated glycoproteins. In: Molecular and Cellular Proteomics. 2009 ; Vol. 8, No. 5. pp. 913-923.
@article{7daa1ac926924f0495a5df1d068bb397,
title = "A strategy for precise and large scale identification of core fucosylated glycoproteins",
abstract = "Core fucosylation (CF) patterns of some glycoproteins are more sensitive and specific than evaluation of their total respective protein levels for diagnosis of many diseases, such as cancers. Global profiling and quantitative characterization of CF glycoproteins may reveal potent biomarkers for clinical applications. However, current techniques are unable to reveal CF glycoproteins precisely on a large scale. Here we developed a robust strategy that integrates molecular weight cutoff, neutral loss-dependent MS3, database-independent candidate spectrum filtering, and optimization to effectively identify CF glycoproteins. The rationale for spectrum treatment was innovatively based on computation of the mass distribution in spectra of CF glycopeptides. The efficacy of this strategy was demonstrated by implementation for plasma from healthy subjects and subjects with hepatocellular carcinoma. Over 100 CF glycoproteins and CF sites were identified, and over 10,000 mass spectra of CF glycopeptide were found. The scale of identification results indicates great progress for finding biomarkers with a particular and attractive prospect, and the candidate spectra will be a useful resource for the improvement of database searching methods for glycopeptides.",
author = "Wei Jia and Zhuang Lu and Yan Fu and Wang, {Hai Peng} and Wang, {Le Heng} and Hao Chi and Yuan, {Zuo Fei} and Zheng, {Zhao Bin} and Song, {Li Na} and Han, {Huan Huan} and Liang, {Yi Min} and Wang, {Jing Lan} and Yun Cai and Zhang, {Yu Kui} and Deng, {Yu Lin} and Ying, {Wan Tao} and He, {Si Min} and Qian, {Xiao Hong}",
year = "2009",
month = may,
doi = "10.1074/mcp.M800504-MCP200",
language = "English",
volume = "8",
pages = "913--923",
journal = "Molecular and Cellular Proteomics",
issn = "1535-9476",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "5",
}
Jia, W, Lu, Z, Fu, Y, Wang, HP, Wang, LH, Chi, H, Yuan, ZF, Zheng, ZB, Song, LN, Han, HH, Liang, YM, Wang, JL, Cai, Y, Zhang, YK, Deng, YL, Ying, WT, He, SM & Qian, XH 2009, 'A strategy for precise and large scale identification of core fucosylated glycoproteins', Molecular and Cellular Proteomics, vol. 8, no. 5, pp. 913-923. https://doi.org/10.1074/mcp.M800504-MCP200
A strategy for precise and large scale identification of core fucosylated glycoproteins. / Jia, Wei; Lu, Zhuang; Fu, Yan et al.
In:
Molecular and Cellular Proteomics, Vol. 8, No. 5, 05.2009, p. 913-923.
Research output: Contribution to journal › Article › peer-review
TY - JOUR
T1 - A strategy for precise and large scale identification of core fucosylated glycoproteins
AU - Jia, Wei
AU - Lu, Zhuang
AU - Fu, Yan
AU - Wang, Hai Peng
AU - Wang, Le Heng
AU - Chi, Hao
AU - Yuan, Zuo Fei
AU - Zheng, Zhao Bin
AU - Song, Li Na
AU - Han, Huan Huan
AU - Liang, Yi Min
AU - Wang, Jing Lan
AU - Cai, Yun
AU - Zhang, Yu Kui
AU - Deng, Yu Lin
AU - Ying, Wan Tao
AU - He, Si Min
AU - Qian, Xiao Hong
PY - 2009/5
Y1 - 2009/5
N2 - Core fucosylation (CF) patterns of some glycoproteins are more sensitive and specific than evaluation of their total respective protein levels for diagnosis of many diseases, such as cancers. Global profiling and quantitative characterization of CF glycoproteins may reveal potent biomarkers for clinical applications. However, current techniques are unable to reveal CF glycoproteins precisely on a large scale. Here we developed a robust strategy that integrates molecular weight cutoff, neutral loss-dependent MS3, database-independent candidate spectrum filtering, and optimization to effectively identify CF glycoproteins. The rationale for spectrum treatment was innovatively based on computation of the mass distribution in spectra of CF glycopeptides. The efficacy of this strategy was demonstrated by implementation for plasma from healthy subjects and subjects with hepatocellular carcinoma. Over 100 CF glycoproteins and CF sites were identified, and over 10,000 mass spectra of CF glycopeptide were found. The scale of identification results indicates great progress for finding biomarkers with a particular and attractive prospect, and the candidate spectra will be a useful resource for the improvement of database searching methods for glycopeptides.
AB - Core fucosylation (CF) patterns of some glycoproteins are more sensitive and specific than evaluation of their total respective protein levels for diagnosis of many diseases, such as cancers. Global profiling and quantitative characterization of CF glycoproteins may reveal potent biomarkers for clinical applications. However, current techniques are unable to reveal CF glycoproteins precisely on a large scale. Here we developed a robust strategy that integrates molecular weight cutoff, neutral loss-dependent MS3, database-independent candidate spectrum filtering, and optimization to effectively identify CF glycoproteins. The rationale for spectrum treatment was innovatively based on computation of the mass distribution in spectra of CF glycopeptides. The efficacy of this strategy was demonstrated by implementation for plasma from healthy subjects and subjects with hepatocellular carcinoma. Over 100 CF glycoproteins and CF sites were identified, and over 10,000 mass spectra of CF glycopeptide were found. The scale of identification results indicates great progress for finding biomarkers with a particular and attractive prospect, and the candidate spectra will be a useful resource for the improvement of database searching methods for glycopeptides.
UR - http://www.scopus.com/inward/record.url?scp=67449100987&partnerID=8YFLogxK
U2 - 10.1074/mcp.M800504-MCP200
DO - 10.1074/mcp.M800504-MCP200
M3 - Article
C2 - 19139490
AN - SCOPUS:67449100987
SN - 1535-9476
VL - 8
SP - 913
EP - 923
JO - Molecular and Cellular Proteomics
JF - Molecular and Cellular Proteomics
IS - 5
ER -
Jia W, Lu Z, Fu Y, Wang HP, Wang LH, Chi H et al. A strategy for precise and large scale identification of core fucosylated glycoproteins. Molecular and Cellular Proteomics. 2009 May;8(5):913-923. doi: 10.1074/mcp.M800504-MCP200