Abstract
A modified indium tin oxide (ITO) microelectrode detector integrated with a dual-channel polydimethylsiloxane (PDMS) microchip is proposed for in-channel amperometric detection in microchip electrophoresis (ME). The gold nanoparticle (GNP)-modified ITO-coated poly-ethylene terephthalate film electrode was fabricated via a photolithography process and direct electrochemical deposition. This electrode fabrication method reduced the cost of this microchip a lot. The electroplating conditions for the GNP-modified ITO electrode were optimized through evaluating the effect on the detection sensitivity and separation efficiency of a dual-channel ME system. Under the optimal conditions, the performance of the new ME amperometric detection system has been demonstrated by analyzing a mixture of p-aminophenol, o-aminophenol and m-aminophenol, which achieved the detection limits of 0.24 μM, 0.41 μM and 0.42 μM, respectively. Finally, the successful detection of antibiotics demonstrated the potential of this dual-channel microchip system for clinical application.
| Original language | English |
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| Pages (from-to) | 4319-4326 |
| Number of pages | 8 |
| Journal | Analytical Methods |
| Volume | 9 |
| Issue number | 29 |
| DOIs | |
| Publication status | Published - 7 Aug 2017 |
| Externally published | Yes |