分子印迹空心球在蛋白质固相萃取中的应用

Translated title of the contribution: Molecularly Imprinted Hollow Spheres for the Solid Phase Extraction of Protein

Zihui Meng, Yifei Wang, Tengsheng Xie, Wei Chen, Lili Qiu, Min Xue*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Molecularly imprinted polymer was widely used as the stuffing of solid phase extraction(SPE) due to its high selectivity. Hb isolation from complex matrix was established based on molecularly imprinted solid phase extraction(MISPE). Mono-dispersed molecularly imprinted hollow spheres(MIHS) of hemoglobin(Hb) were synthesized using silica nanospheres as the sacrificial matrix. It is proved that the MIHS with good monodispersion and uniform particle size by TEM scanning was suitable for SPE enrichment matrix. Therefore, the extraction of Hb, bovine serum albumin(BSA) and cytochrome C(Cyt C) by MIHSs was investigated by SPE combined with liquid chromatographic method. The recovery of Hb was higher than 80% in phosphate buffer solution(pH=7) while it was lower than 50% for RNase A and Cyt C. Meanwhile, the degree of precision(RSD) was lower than 5%. Compared to the surface imprinted silica(SIS), the MIHS had good recognition and fast adsorption-desorption dynamics for Hb. Moreover, this method was also confirmed in real sample, and the adsorption of Hb was 82% in the spiked bovine serum.

Translated title of the contributionMolecularly Imprinted Hollow Spheres for the Solid Phase Extraction of Protein
Original languageChinese (Traditional)
Pages (from-to)62-68
Number of pages7
JournalKao Teng Hsueh Hsiao Hua Heush Hsueh Pao/ Chemical Journal of Chinese Universities
Volume40
Issue number1
DOIs
Publication statusPublished - 1 Jan 2019

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