全脑辐照致神经损伤大鼠神经化学物质类生物标志物的筛选

Screening of neurochemical biomarkers was performed in peripheral blood serum from rats with nerve injury induced by whole-brain irradiation(WBI).Serum of rats treated with different doses of WBI(0, 10, and 30 Gy) and fractional cumulative radiation(10 Gy×3) were determined for the concentration of 42 neurochemicals using the previously established chemical derivatization and ultra-high performance liquid chromatography-tandem mass spectrometry method followed by statistical analysis based on targeted metabolomics. Univariate statistical analysis indicated that the concentrations of norepinephrine, acetylcholine, γ-aminobutyric acid, tyramine, glutamic acid, tyrosine, tryptophan, melatonin, α-alanine, and glycine were significantly (p<0.05) lower than the control group (0 Gy), while the concentrations of acetylcholine and melatonin changed in the opposite trend (p<0.05); Meanwhile the variation degree of neurochemicals in the 10 Gy×3 group was found lower than that in the 30 Gy group, indicating that the disturbance degree of neurochemicals caused by cumulative irradiation was lower than that caused by single irradiation of the same dose. Combining with the variable importance in the projection(VIP)in the multivariate statistical OPLS-DA model, the proposed method has selected acetylcholine, glutamate, tyramine, and melatonin as the potential biomarkers for the nerve injury induced by WBI.