TY - GEN
T1 - Using electrically tunable lens to improve axial resolution and imaging field in light sheet fluorescence microscope
AU - Zhai, Muyue
AU - Huang, Xiaoshuai
AU - Mao, Heng
AU - Zhu, Qiudong
AU - Wang, Shanshan
N1 - Publisher Copyright:
© Springer International Publishing AG, part of Springer Nature 2019.
PY - 2019
Y1 - 2019
N2 - Light sheet fluorescence microscope (LSFM) has become one of the most promising 3D microscopy techniques due to its prominent lateral section illumination. In addition, comparing with the confocal microscope, it has low phototoxicity and high temporal resolution. In this paper, we proposed an easy-to-operate tiling light sheet fluorescence microscope, which integrates a cylindrical lens, a galvanometric mirror, and an excitation objective to produce a light sheet with desired numerical aperture and axial resolution and utilizes an electrically tunable lens (ETL) to enlarge the limited field of view (FOV) of single excitation section following the temporal multiplexing. Imaging performances have been characterized from the experiment by imaging the 20 nm fluorescent beads with water immersion, and the benefits of our proposed technique have been shown in volumetric resolution, FOV, and speed as well as contrast.
AB - Light sheet fluorescence microscope (LSFM) has become one of the most promising 3D microscopy techniques due to its prominent lateral section illumination. In addition, comparing with the confocal microscope, it has low phototoxicity and high temporal resolution. In this paper, we proposed an easy-to-operate tiling light sheet fluorescence microscope, which integrates a cylindrical lens, a galvanometric mirror, and an excitation objective to produce a light sheet with desired numerical aperture and axial resolution and utilizes an electrically tunable lens (ETL) to enlarge the limited field of view (FOV) of single excitation section following the temporal multiplexing. Imaging performances have been characterized from the experiment by imaging the 20 nm fluorescent beads with water immersion, and the benefits of our proposed technique have been shown in volumetric resolution, FOV, and speed as well as contrast.
KW - Electrically tunable lens
KW - High axial resolution
KW - Large field of view
KW - Light sheet fluorescence microscope
UR - https://www.scopus.com/pages/publications/85054379079
U2 - 10.1007/978-3-319-91659-0_34
DO - 10.1007/978-3-319-91659-0_34
M3 - Conference contribution
AN - SCOPUS:85054379079
SN - 9783319916583
T3 - Lecture Notes in Electrical Engineering
SP - 411
EP - 419
BT - Proceedings of the International Conference on Sensing and Imaging
A2 - Quinto, Eric Todd
A2 - Ida, Nathan
A2 - Louis, Alfred K.
A2 - Jiang, Ming
PB - Springer Verlag
T2 - International Conference on Sensing and Imaging, ICSI 2017
Y2 - 5 June 2017 through 7 June 2017
ER -