The transcriptional coactivator RUVBL2 regulates Pol II clustering with diverse transcription factors

Hui Wang; Boyuan Li; Linyu Zuo; Bo Wang; Yan Yan; Kai Tian; Rong Zhou; Chenlu Wang; Xizi Chen; Yongpeng Jiang; Haonan Zheng; Fangfei Qin; Bin Zhang; Yang Yu; Chao Pei Liu; Yanhui Xu; Juntao Gao; Zhi Qi; Wulan Deng; Xiong Ji

doi:10.1038/s41467-022-33433-3

The transcriptional coactivator RUVBL2 regulates Pol II clustering with diverse transcription factors

Hui Wang
, Boyuan Li
, Linyu Zuo
, Bo Wang
, Yan Yan
, Kai Tian
, Rong Zhou
, Chenlu Wang
, Xizi Chen
, Yongpeng Jiang
, Haonan Zheng
, Fangfei Qin
, Bin Zhang
, Yang Yu
, Chao Pei Liu
, Yanhui Xu
, Juntao Gao
, Zhi Qi
, Wulan Deng
, Xiong Ji^*

^*此作品的通讯作者

Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education
Peking University
Department of Pathogenic Biology
Chengdu Medical College
Center for Quantitative Biology
Biomedical Pioneering Innovation Center (BIOPIC)
Tsinghua University
Center for Synthetic and Systems Biology
Institutes of Biomedical Sciences
Fudan University
Synthetic and Functional Biomolecules Center
Departments of Pathology and Laboratory Medicine
University of Rochester
Key Laboratory of RNA Biology
CAS - Institute of Biophysics
National Laboratory of Biomacromolecules

科研成果: 期刊稿件 › 文章 › 同行评审

40 引用（Scopus）

摘要

RNA polymerase II (Pol II) apparatuses are compartmentalized into transcriptional clusters. Whether protein factors control these clusters remains unknown. In this study, we find that the ATPase-associated with diverse cellular activities (AAA +) ATPase RUVBL2 co-occupies promoters with Pol II and various transcription factors. RUVBL2 interacts with unphosphorylated Pol II in chromatin to promote RPB1 carboxy-terminal domain (CTD) clustering and transcription initiation. Rapid depletion of RUVBL2 leads to a decrease in the number of Pol II clusters and inhibits nascent RNA synthesis, and tethering RUVBL2 to an active promoter enhances Pol II clustering at the promoter. We also identify target genes that are directly linked to the RUVBL2-Pol II axis. Many of these genes are hallmarks of cancers and encode proteins with diverse cellular functions. Our results demonstrate an emerging activity for RUVBL2 in regulating Pol II cluster formation in the nucleus.

源语言	英语
期刊论文编号	5703
期刊	Nature Communications
卷	13
期	1
DOI	https://doi.org/10.1038/s41467-022-33433-3
出版状态	已出版 - 12月 2022
已对外发布	是

联合国可持续发展目标

此成果有助于实现下列可持续发展目标：

可持续发展目标 3 良好健康与福祉

访问文件

10.1038/s41467-022-33433-3

其它文件与链接

链接到 Scopus 的出版物

引用此

Wang, H., Li, B., Zuo, L., Wang, B., Yan, Y., Tian, K., Zhou, R., Wang, C., Chen, X., Jiang, Y., Zheng, H., Qin, F., Zhang, B., Yu, Y., Liu, C. P., Xu, Y., Gao, J., Qi, Z., Deng, W., & Ji, X. (2022). The transcriptional coactivator RUVBL2 regulates Pol II clustering with diverse transcription factors. Nature Communications , 13(1), 期刊论文 5703. https://doi.org/10.1038/s41467-022-33433-3

@article{d0b85a553efd44c286bd6f1c621f0a3f,

title = "The transcriptional coactivator RUVBL2 regulates Pol II clustering with diverse transcription factors",

abstract = "RNA polymerase II (Pol II) apparatuses are compartmentalized into transcriptional clusters. Whether protein factors control these clusters remains unknown. In this study, we find that the ATPase-associated with diverse cellular activities (AAA +) ATPase RUVBL2 co-occupies promoters with Pol II and various transcription factors. RUVBL2 interacts with unphosphorylated Pol II in chromatin to promote RPB1 carboxy-terminal domain (CTD) clustering and transcription initiation. Rapid depletion of RUVBL2 leads to a decrease in the number of Pol II clusters and inhibits nascent RNA synthesis, and tethering RUVBL2 to an active promoter enhances Pol II clustering at the promoter. We also identify target genes that are directly linked to the RUVBL2-Pol II axis. Many of these genes are hallmarks of cancers and encode proteins with diverse cellular functions. Our results demonstrate an emerging activity for RUVBL2 in regulating Pol II cluster formation in the nucleus.",

author = "Hui Wang and Boyuan Li and Linyu Zuo and Bo Wang and Yan Yan and Kai Tian and Rong Zhou and Chenlu Wang and Xizi Chen and Yongpeng Jiang and Haonan Zheng and Fangfei Qin and Bin Zhang and Yang Yu and Liu, \{Chao Pei\} and Yanhui Xu and Juntao Gao and Zhi Qi and Wulan Deng and Xiong Ji",

note = "Publisher Copyright: {\textcopyright} 2022, The Author(s).",

year = "2022",

month = dec,

doi = "10.1038/s41467-022-33433-3",

language = "English",

volume = "13",

journal = "Nature Communications ",

issn = "2041-1723",

number = "1",

}

Wang, H, Li, B, Zuo, L, Wang, B, Yan, Y, Tian, K, Zhou, R, Wang, C, Chen, X, Jiang, Y, Zheng, H, Qin, F, Zhang, B, Yu, Y, Liu, CP, Xu, Y, Gao, J, Qi, Z, Deng, W & Ji, X 2022, 'The transcriptional coactivator RUVBL2 regulates Pol II clustering with diverse transcription factors', Nature Communications , 卷 13, 号码 1, 5703. https://doi.org/10.1038/s41467-022-33433-3

TY - JOUR

T1 - The transcriptional coactivator RUVBL2 regulates Pol II clustering with diverse transcription factors

AU - Wang, Hui

AU - Li, Boyuan

AU - Zuo, Linyu

AU - Wang, Bo

AU - Yan, Yan

AU - Tian, Kai

AU - Zhou, Rong

AU - Wang, Chenlu

AU - Chen, Xizi

AU - Jiang, Yongpeng

AU - Zheng, Haonan

AU - Qin, Fangfei

AU - Zhang, Bin

AU - Yu, Yang

AU - Liu, Chao Pei

AU - Xu, Yanhui

AU - Gao, Juntao

AU - Qi, Zhi

AU - Deng, Wulan

AU - Ji, Xiong

PY - 2022/12

Y1 - 2022/12

N2 - RNA polymerase II (Pol II) apparatuses are compartmentalized into transcriptional clusters. Whether protein factors control these clusters remains unknown. In this study, we find that the ATPase-associated with diverse cellular activities (AAA +) ATPase RUVBL2 co-occupies promoters with Pol II and various transcription factors. RUVBL2 interacts with unphosphorylated Pol II in chromatin to promote RPB1 carboxy-terminal domain (CTD) clustering and transcription initiation. Rapid depletion of RUVBL2 leads to a decrease in the number of Pol II clusters and inhibits nascent RNA synthesis, and tethering RUVBL2 to an active promoter enhances Pol II clustering at the promoter. We also identify target genes that are directly linked to the RUVBL2-Pol II axis. Many of these genes are hallmarks of cancers and encode proteins with diverse cellular functions. Our results demonstrate an emerging activity for RUVBL2 in regulating Pol II cluster formation in the nucleus.

AB - RNA polymerase II (Pol II) apparatuses are compartmentalized into transcriptional clusters. Whether protein factors control these clusters remains unknown. In this study, we find that the ATPase-associated with diverse cellular activities (AAA +) ATPase RUVBL2 co-occupies promoters with Pol II and various transcription factors. RUVBL2 interacts with unphosphorylated Pol II in chromatin to promote RPB1 carboxy-terminal domain (CTD) clustering and transcription initiation. Rapid depletion of RUVBL2 leads to a decrease in the number of Pol II clusters and inhibits nascent RNA synthesis, and tethering RUVBL2 to an active promoter enhances Pol II clustering at the promoter. We also identify target genes that are directly linked to the RUVBL2-Pol II axis. Many of these genes are hallmarks of cancers and encode proteins with diverse cellular functions. Our results demonstrate an emerging activity for RUVBL2 in regulating Pol II cluster formation in the nucleus.

UR - https://www.scopus.com/pages/publications/85138864251

U2 - 10.1038/s41467-022-33433-3

DO - 10.1038/s41467-022-33433-3

M3 - Article

C2 - 36171202

AN - SCOPUS:85138864251

SN - 2041-1723

VL - 13

JO - Nature Communications

JF - Nature Communications

IS - 1

M1 - 5703

ER -

The transcriptional coactivator RUVBL2 regulates Pol II clustering with diverse transcription factors

摘要

联合国可持续发展目标

访问文件

其它文件与链接

学术指纹

引用此