The synthesis and characterization of β-cyclodextrin derivated pancreatin used as chiral selector in capillary electrochromatography

The preparation of capillary electrochromatography column for chiral separation with β-cyclo-dextrin derivated pancreatin was investigated, which had three steps, synthesis of β-cyclodextrin derivated pancreatin, the modification of the capillary internal surface and the covalent binding of β-cyclodextrin derivated pancreatin on the capillary internal wall. β-cyclodextrin derivated pancreatin was synthesized using β-cyclodextrin and protein in the presence of ethylene glycol diglycidyl ether (EGDE) in boric acid buffer at pH = 8.7. Amino group was coated on the internal surface of the silica capillary by sol-gel technology using triethoxylmethylsiloxane and (3-aminopropyl) trimethoxysiloxane. Covalent binding of β-cyclodextrin derivated pancreatin was performed by glutaraldehyde. Chlorpheniramine, phenylalanine and troopine were separated baselines by β-cyclodextrin derivated pancreatin in capillary electrochromatography.