TY - JOUR
T1 - The method of immobilized pH gradient of CIEF used in protein separation
AU - Xie, Yao
AU - Geng, Lina
AU - Qu, Feng
AU - Luo, Aiqin
AU - Deng, Yulin
PY - 2008
Y1 - 2008
N2 - An immobilized pH gradient method had been developed for proteins separation by two-step capillary isoelectric focusing based on chemical bonding. The double functional reagent, N,N′-carbonyldiimidazole was introduced in the capillary as a linkage in the immobilization reactions. Moreover, the commercial ampholytes (CAs) were distributed according to their pI values in the condition of electric field, and then connected with N,N′- carbonyldiimidazole by chemical bonding to realize the immobilized pH gradient in capillary. A test sample containing a protein mixture of hemoglobin (Hb), bovine serum albumin (BSA) and pepsin was separated by using this method.
AB - An immobilized pH gradient method had been developed for proteins separation by two-step capillary isoelectric focusing based on chemical bonding. The double functional reagent, N,N′-carbonyldiimidazole was introduced in the capillary as a linkage in the immobilization reactions. Moreover, the commercial ampholytes (CAs) were distributed according to their pI values in the condition of electric field, and then connected with N,N′- carbonyldiimidazole by chemical bonding to realize the immobilized pH gradient in capillary. A test sample containing a protein mixture of hemoglobin (Hb), bovine serum albumin (BSA) and pepsin was separated by using this method.
KW - Capillary isoelectric focusing
KW - Immobilized pH gradient
KW - N,N′-carbonyldiimidazole
KW - Protein separation
UR - https://www.scopus.com/pages/publications/50049106466
M3 - Article
AN - SCOPUS:50049106466
SN - 0441-3776
VL - 71
SP - 461
EP - 464
JO - Chemistry Bulletin / Huaxue Tongbao
JF - Chemistry Bulletin / Huaxue Tongbao
IS - 6
ER -