Surface plasmon resonance imaging sensor based on hue algorithm

A Surface Plasmon Resonance Imaging sensor based on hue algorithm has been presented. The sensor can be used for observing the chemical or physical reactions on the surface of the SPR chip. What is more, the hue algorithm can be used to analyze these reactions on the surface of the SPR chip quantitatively. First of all, the wavelength/image synchronous detection SPR sensor was used to obtain the resonance spectra with different resonance wavelengths and the corresponding color images. And then, the hue algorithm was used to get the hue distribution of the image and determine the relationship between the resonance wavelengths and the average hue. Considering the relationship between the average hue and the resonance wavelengths, the initial resonance wavelength was set as 650 nm. Under this circumstance, the sensitivity of the hue is about 3 338/RIU, which is 1.49 times as the sensitivity of the resonance wavelength. The Teflon layer was fabricated on the gold film, and the thickness of the layer was analyzed by the color SPRI image. The hue of the local areas was calculated to determine the sensitivity of the different thicknesses of the Teflon layer quantitatively. The experimental results indicate that the SPRI sensor based on hue algorithm is better than the conventional SPR sensor distinctly.