Streptomyces wuyuanensis sp. nov., an actinomycete from soil

A novel actinomycete, strain FX61^T, was isolated from a saline sample collected from the Inner Mongolian Autonomous Region in China and subjected to a taxonomic study using a polyphasic approach. The predominant menaquinones were MK-9(H₆), MK-9(H₈) andMK-9(H₄). The major fatty acids were iso-C_{16: 0}, anteiso-C_{15: 0}, iso-C_{15: 0}, iso-C_{16: 1} H,C_16:0, iso-C_{14: 0} and anteiso-C_17:0. The phospholipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, two phosphatidylinositol mannosides and an unidentified phospholipid. The G+C content of the genomic DNA was 72 mol%. The 16S rRNA gene sequence of the isolate had greater than 98%similarity with those of Streptomyces griseoincarnatus ATCC 23623^T (98.2 %), Streptomyces labedae DSM 41446^T (98.2 %), Streptomyces variabilis ATCC 19815^T (98.2 %), Streptomyces erythrogriseus ATCC 27427^T (98.2%), Streptomyces matensis ATCC 23935^T (98.2 %), Streptomyces althioticus ATCC 19724^T (98.2 %) and Streptomyces luteosporeus ATCC 33049^T (98.0 %), showing that the novel strain should be assigned to the genus Streptomyces. DNA- DNA hybridizations with the seven above-mentioned members of the genus Streptomyces showed 29.8, 28.5, 27.0, 25.5, 25.0, 23.5 and 22.0%relatedness, respectively. On the basis of the phenotypic characteristics and genotypic distinctiveness, strain FX61^T should be classified as a novel species of the genus Streptomyces, for which the name Streptomyces wuyuanensis sp. nov. is proposed. The type strain is FX61^T (=CGMCC 4.7042^T=KCTC 29112^T).