TY - JOUR
T1 - Streptomyces lutosisoli sp. nov., a novel actinomycete isolated from muddy soil
AU - Shen, Yue
AU - Sun, Tianyu
AU - Jiang, Shanwen
AU - Mu, Shan
AU - Li, Dongmei
AU - Guo, Xiaowei
AU - Zhang, Jianli
AU - Zhao, Junwei
AU - Xiang, Wensheng
N1 - Publisher Copyright:
© 2018, Springer Nature Switzerland AG.
PY - 2018/12/1
Y1 - 2018/12/1
N2 - A novel Gram-stain positive, spore-forming, aerobic actinomycete, designated strain NEAU-QTH3-11 T , was isolated from muddy soil collected from a stream in Qitaihe, Heilongjiang Province, northeast China and characterised using a polyphasic approach. The 16S rRNA gene sequence analysis showed that strain NEAU-QTH3-11 T belongs to the genus Streptomyces and is closely related to Streptomyces rhizosphaerihabitans NBRC 109807 T (99.38%) and Streptomyces mirabilis JCM 4791 T (99.03%). Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the strain formed a cluster with S. rhizosphaerihabitans NBRC 109807 T and Streptomyces siamensis NBRC 108799 T (98.62%). The menaquinones were identified as MK-9(H 8 ), MK-9(H 6 ) and MK-9(H 4 ). The phospholipid profile was found to consist of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, an unidentified phospholipid and an unidentified lipid. The major fatty acids were identified as anteiso-C 15:0 , iso-C 16:0 , C 16:0 and C 15:0 . However, multilocus sequence analysis based on five house-keeping genes (atpD, gyrB, rpoB, recA and trpB), low DNA-DNA hybridization results and some phenotypic, physiological and biochemical properties could differentiate the strain from its close relatives in the genus Streptomyces. Therefore, strain NEAU-QTH3-11 T is considered to represent a novel species of the genus Streptomyces, for which the name Streptomyces lutosisoli sp. nov. is proposed, with NEAU-QTH3-11 T (=DSM 42165 T =CGMCC 4.7198 T ) as the type strain.
AB - A novel Gram-stain positive, spore-forming, aerobic actinomycete, designated strain NEAU-QTH3-11 T , was isolated from muddy soil collected from a stream in Qitaihe, Heilongjiang Province, northeast China and characterised using a polyphasic approach. The 16S rRNA gene sequence analysis showed that strain NEAU-QTH3-11 T belongs to the genus Streptomyces and is closely related to Streptomyces rhizosphaerihabitans NBRC 109807 T (99.38%) and Streptomyces mirabilis JCM 4791 T (99.03%). Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the strain formed a cluster with S. rhizosphaerihabitans NBRC 109807 T and Streptomyces siamensis NBRC 108799 T (98.62%). The menaquinones were identified as MK-9(H 8 ), MK-9(H 6 ) and MK-9(H 4 ). The phospholipid profile was found to consist of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, an unidentified phospholipid and an unidentified lipid. The major fatty acids were identified as anteiso-C 15:0 , iso-C 16:0 , C 16:0 and C 15:0 . However, multilocus sequence analysis based on five house-keeping genes (atpD, gyrB, rpoB, recA and trpB), low DNA-DNA hybridization results and some phenotypic, physiological and biochemical properties could differentiate the strain from its close relatives in the genus Streptomyces. Therefore, strain NEAU-QTH3-11 T is considered to represent a novel species of the genus Streptomyces, for which the name Streptomyces lutosisoli sp. nov. is proposed, with NEAU-QTH3-11 T (=DSM 42165 T =CGMCC 4.7198 T ) as the type strain.
KW - 16S rRNA gene
KW - Polyphasic taxonomy
KW - Streptomyces lutosisoli sp. nov
UR - https://www.scopus.com/pages/publications/85049688628
U2 - 10.1007/s10482-018-1129-2
DO - 10.1007/s10482-018-1129-2
M3 - Article
C2 - 29995193
AN - SCOPUS:85049688628
SN - 0003-6072
VL - 111
SP - 2403
EP - 2412
JO - Antonie van Leeuwenhoek
JF - Antonie van Leeuwenhoek
IS - 12
ER -