Single cell atlas for 11 non-model mammals, reptiles and birds

Dongsheng Chen; Jian Sun; Jiacheng Zhu; Xiangning Ding; Tianming Lan; Xiran Wang; Weiying Wu; Zhihua Ou; Linnan Zhu; Peiwen Ding; Haoyu Wang; Lihua Luo; Rong Xiang; Xiaoling Wang; Jiaying Qiu; Shiyou Wang; Haimeng Li; Chaochao Chai; Langchao Liang; Fuyu An; Le Zhang; Lei Han; Yixin Zhu; Feiyue Wang; Yuting Yuan; Wendi Wu; Chengcheng Sun; Haorong Lu; Jihong Wu; Xinghuai Sun; Shenghai Zhang; Sunil Kumar Sahu; Ping Liu; Jun Xia; Lijing Zhang; Haixia Chen; Dongming Fang; Yuying Zeng; Yiquan Wu; Zehua Cui; Qian He; Sanjie Jiang; Xiaoyan Ma; Weimin Feng; Yan Xu; Fang Li; Zhongmin Liu; Lei Chen; Fang Chen; Xin Jin; Wei Qiu; Tianjiao Wang; Yang Li; Xiumei Xing; Huanming Yang; Yanchun Xu; Yan Hua; Yahong Liu; Huan Liu; Xun Xu

doi:10.1038/s41467-021-27162-2

Single cell atlas for 11 non-model mammals, reptiles and birds

Dongsheng Chen
, Jian Sun
, Jiacheng Zhu
, Xiangning Ding
, Tianming Lan
, Xiran Wang
, Weiying Wu
, Zhihua Ou
, Linnan Zhu
, Peiwen Ding
, Haoyu Wang
, Lihua Luo
, Rong Xiang
, Xiaoling Wang
, Jiaying Qiu
, Shiyou Wang
, Haimeng Li
, Chaochao Chai
, Langchao Liang
, Fuyu An

Le Zhang, Lei Han, Yixin Zhu, Feiyue Wang, Yuting Yuan, Wendi Wu, Chengcheng Sun, Haorong Lu, Jihong Wu, Xinghuai Sun, Shenghai Zhang, Sunil Kumar Sahu, Ping Liu, Jun Xia, Lijing Zhang, Haixia Chen, Dongming Fang, Yuying Zeng, Yiquan Wu, Zehua Cui, Qian He, Sanjie Jiang, Xiaoyan Ma, Weimin Feng, Yan Xu, Fang Li, Zhongmin Liu, Lei Chen, Fang Chen, Xin Jin, Wei Qiu, Tianjiao Wang, Yang Li, Xiumei Xing, Huanming Yang, Yanchun Xu, Yan Hua^*, Yahong Liu^*, Huan Liu^*, Xun Xu^*

^*此作品的通讯作者

BGI-Shenzhen
South China Agricultural University
Guangdong Laboratory for Lingnan Modern Agriculture
University of Chinese Academy of Sciences
University of Copenhagen
Guangdong Academy of Forestry
College of Wildlife Resources Northeast Forestry University
Fudan University
Science and Technology Commission of Shanghai Municipality
Ministry of Health of People's Republic of China
National Institutes of Health
University of Cambridge
Tongji University
Yangzhou University
The Third Affiliated Hospital of Sun Yat-sen University
Chinese Academy of Agricultural Sciences

科研成果: 期刊稿件 › 文章 › 同行评审

48 引用（Scopus）

摘要

The availability of viral entry factors is a prerequisite for the cross-species transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Large-scale single-cell screening of animal cells could reveal the expression patterns of viral entry genes in different hosts. However, such exploration for SARS-CoV-2 remains limited. Here, we perform single-nucleus RNA sequencing for 11 non-model species, including pets (cat, dog, hamster, and lizard), livestock (goat and rabbit), poultry (duck and pigeon), and wildlife (pangolin, tiger, and deer), and investigated the co-expression of ACE2 and TMPRSS2. Furthermore, cross-species analysis of the lung cell atlas of the studied mammals, reptiles, and birds reveals core developmental programs, critical connectomes, and conserved regulatory circuits among these evolutionarily distant species. Overall, our work provides a compendium of gene expression profiles for non-model animals, which could be employed to identify potential SARS-CoV-2 target cells and putative zoonotic reservoirs.

源语言	英语
文章编号	7083
期刊	Nature Communications
卷	12
期	1
DOI	https://doi.org/10.1038/s41467-021-27162-2
出版状态	已出版 - 12月 2021
已对外发布	是

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@article{23c25278f27347b69ae3319dc8b3701e,

title = "Single cell atlas for 11 non-model mammals, reptiles and birds",

abstract = "The availability of viral entry factors is a prerequisite for the cross-species transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Large-scale single-cell screening of animal cells could reveal the expression patterns of viral entry genes in different hosts. However, such exploration for SARS-CoV-2 remains limited. Here, we perform single-nucleus RNA sequencing for 11 non-model species, including pets (cat, dog, hamster, and lizard), livestock (goat and rabbit), poultry (duck and pigeon), and wildlife (pangolin, tiger, and deer), and investigated the co-expression of ACE2 and TMPRSS2. Furthermore, cross-species analysis of the lung cell atlas of the studied mammals, reptiles, and birds reveals core developmental programs, critical connectomes, and conserved regulatory circuits among these evolutionarily distant species. Overall, our work provides a compendium of gene expression profiles for non-model animals, which could be employed to identify potential SARS-CoV-2 target cells and putative zoonotic reservoirs.",

author = "Dongsheng Chen and Jian Sun and Jiacheng Zhu and Xiangning Ding and Tianming Lan and Xiran Wang and Weiying Wu and Zhihua Ou and Linnan Zhu and Peiwen Ding and Haoyu Wang and Lihua Luo and Rong Xiang and Xiaoling Wang and Jiaying Qiu and Shiyou Wang and Haimeng Li and Chaochao Chai and Langchao Liang and Fuyu An and Le Zhang and Lei Han and Yixin Zhu and Feiyue Wang and Yuting Yuan and Wendi Wu and Chengcheng Sun and Haorong Lu and Jihong Wu and Xinghuai Sun and Shenghai Zhang and Sahu, \{Sunil Kumar\} and Ping Liu and Jun Xia and Lijing Zhang and Haixia Chen and Dongming Fang and Yuying Zeng and Yiquan Wu and Zehua Cui and Qian He and Sanjie Jiang and Xiaoyan Ma and Weimin Feng and Yan Xu and Fang Li and Zhongmin Liu and Lei Chen and Fang Chen and Xin Jin and Wei Qiu and Tianjiao Wang and Yang Li and Xiumei Xing and Huanming Yang and Yanchun Xu and Yan Hua and Yahong Liu and Huan Liu and Xun Xu",

note = "Publisher Copyright: {\textcopyright} 2021, The Author(s).",

year = "2021",

month = dec,

doi = "10.1038/s41467-021-27162-2",

language = "English",

volume = "12",

journal = "Nature Communications",

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Chen, D, Sun, J, Zhu, J, Ding, X, Lan, T, Wang, X, Wu, W, Ou, Z, Zhu, L, Ding, P, Wang, H, Luo, L, Xiang, R, Wang, X, Qiu, J, Wang, S, Li, H, Chai, C, Liang, L, An, F, Zhang, L, Han, L, Zhu, Y, Wang, F, Yuan, Y, Wu, W, Sun, C, Lu, H, Wu, J, Sun, X, Zhang, S, Sahu, SK, Liu, P, Xia, J, Zhang, L, Chen, H, Fang, D, Zeng, Y, Wu, Y, Cui, Z, He, Q, Jiang, S, Ma, X, Feng, W, Xu, Y, Li, F, Liu, Z, Chen, L, Chen, F, Jin, X, Qiu, W, Wang, T, Li, Y, Xing, X, Yang, H, Xu, Y, Hua, Y, Liu, Y, Liu, H & Xu, X 2021, 'Single cell atlas for 11 non-model mammals, reptiles and birds', Nature Communications, 卷 12, 号码 1, 7083. https://doi.org/10.1038/s41467-021-27162-2

TY - JOUR

T1 - Single cell atlas for 11 non-model mammals, reptiles and birds

AU - Chen, Dongsheng

AU - Sun, Jian

AU - Zhu, Jiacheng

AU - Ding, Xiangning

AU - Lan, Tianming

AU - Wang, Xiran

AU - Wu, Weiying

AU - Ou, Zhihua

AU - Zhu, Linnan

AU - Ding, Peiwen

AU - Wang, Haoyu

AU - Luo, Lihua

AU - Xiang, Rong

AU - Wang, Xiaoling

AU - Qiu, Jiaying

AU - Wang, Shiyou

AU - Li, Haimeng

AU - Chai, Chaochao

AU - Liang, Langchao

AU - An, Fuyu

AU - Zhang, Le

AU - Han, Lei

AU - Zhu, Yixin

AU - Wang, Feiyue

AU - Yuan, Yuting

AU - Wu, Wendi

AU - Sun, Chengcheng

AU - Lu, Haorong

AU - Wu, Jihong

AU - Sun, Xinghuai

AU - Zhang, Shenghai

AU - Sahu, Sunil Kumar

AU - Liu, Ping

AU - Xia, Jun

AU - Zhang, Lijing

AU - Chen, Haixia

AU - Fang, Dongming

AU - Zeng, Yuying

AU - Wu, Yiquan

AU - Cui, Zehua

AU - He, Qian

AU - Jiang, Sanjie

AU - Ma, Xiaoyan

AU - Feng, Weimin

AU - Xu, Yan

AU - Li, Fang

AU - Liu, Zhongmin

AU - Chen, Lei

AU - Chen, Fang

AU - Jin, Xin

AU - Qiu, Wei

AU - Wang, Tianjiao

AU - Li, Yang

AU - Xing, Xiumei

AU - Yang, Huanming

AU - Xu, Yanchun

AU - Hua, Yan

AU - Liu, Yahong

AU - Liu, Huan

AU - Xu, Xun

PY - 2021/12

Y1 - 2021/12

N2 - The availability of viral entry factors is a prerequisite for the cross-species transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Large-scale single-cell screening of animal cells could reveal the expression patterns of viral entry genes in different hosts. However, such exploration for SARS-CoV-2 remains limited. Here, we perform single-nucleus RNA sequencing for 11 non-model species, including pets (cat, dog, hamster, and lizard), livestock (goat and rabbit), poultry (duck and pigeon), and wildlife (pangolin, tiger, and deer), and investigated the co-expression of ACE2 and TMPRSS2. Furthermore, cross-species analysis of the lung cell atlas of the studied mammals, reptiles, and birds reveals core developmental programs, critical connectomes, and conserved regulatory circuits among these evolutionarily distant species. Overall, our work provides a compendium of gene expression profiles for non-model animals, which could be employed to identify potential SARS-CoV-2 target cells and putative zoonotic reservoirs.

AB - The availability of viral entry factors is a prerequisite for the cross-species transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Large-scale single-cell screening of animal cells could reveal the expression patterns of viral entry genes in different hosts. However, such exploration for SARS-CoV-2 remains limited. Here, we perform single-nucleus RNA sequencing for 11 non-model species, including pets (cat, dog, hamster, and lizard), livestock (goat and rabbit), poultry (duck and pigeon), and wildlife (pangolin, tiger, and deer), and investigated the co-expression of ACE2 and TMPRSS2. Furthermore, cross-species analysis of the lung cell atlas of the studied mammals, reptiles, and birds reveals core developmental programs, critical connectomes, and conserved regulatory circuits among these evolutionarily distant species. Overall, our work provides a compendium of gene expression profiles for non-model animals, which could be employed to identify potential SARS-CoV-2 target cells and putative zoonotic reservoirs.

UR - https://www.scopus.com/pages/publications/85120879942

U2 - 10.1038/s41467-021-27162-2

DO - 10.1038/s41467-021-27162-2

M3 - Article

C2 - 34873160

AN - SCOPUS:85120879942

SN - 2041-1723

VL - 12

JO - Nature Communications

JF - Nature Communications

IS - 1

M1 - 7083

ER -

Single cell atlas for 11 non-model mammals, reptiles and birds

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