Significant increase of oxidase activity through the genetic incorporation of a tyrosine-histidine cross-link in a myoglobin model of heme-copper oxidase

Xiaohong Liu; Yang Yu; Cheng Hu; Wei Zhang; Yi Lu; Jiangyun Wang

doi:10.1002/anie.201108756

Significant increase of oxidase activity through the genetic incorporation of a tyrosine-histidine cross-link in a myoglobin model of heme-copper oxidase

Xiaohong Liu^*
, Yang Yu
, Cheng Hu
, Wei Zhang
, Yi Lu
, Jiangyun Wang

^*此作品的通讯作者

科研成果: 期刊稿件 › 文章 › 同行评审

108 引用（Scopus）

摘要

Top model: Heme-copper oxidase (HCO) contains a histidine-tyrosine cross-link in its heme a3/Cu _B oxygen reduction center. A functional model of HCO was obtained through the genetic incorporation of the unnatural amino acid imiTyr, which mimics the Tyr-His cross-link, and of the Cu _B site into myoglobin (see picture). Like HCO, this small soluble protein exhibits selective O ₂-reduction activity while generating little reactive oxygen species.

源语言	英语
页（从-至）	4312-4316
页数	5
期刊	Angewandte Chemie - International Edition
卷	51
期	18
DOI	https://doi.org/10.1002/anie.201108756
出版状态	已出版 - 27 4月 2012
已对外发布	是

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title = "Significant increase of oxidase activity through the genetic incorporation of a tyrosine-histidine cross-link in a myoglobin model of heme-copper oxidase",

abstract = "Top model: Heme-copper oxidase (HCO) contains a histidine-tyrosine cross-link in its heme a3/Cu B oxygen reduction center. A functional model of HCO was obtained through the genetic incorporation of the unnatural amino acid imiTyr, which mimics the Tyr-His cross-link, and of the Cu B site into myoglobin (see picture). Like HCO, this small soluble protein exhibits selective O 2-reduction activity while generating little reactive oxygen species.",

keywords = "enzyme catalysis, enzyme models, oxygen reduction, post-translational modifications, protein design",

author = "Xiaohong Liu and Yang Yu and Cheng Hu and Wei Zhang and Yi Lu and Jiangyun Wang",

year = "2012",

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T1 - Significant increase of oxidase activity through the genetic incorporation of a tyrosine-histidine cross-link in a myoglobin model of heme-copper oxidase

AU - Liu, Xiaohong

AU - Yu, Yang

AU - Hu, Cheng

AU - Zhang, Wei

AU - Lu, Yi

AU - Wang, Jiangyun

PY - 2012/4/27

Y1 - 2012/4/27

N2 - Top model: Heme-copper oxidase (HCO) contains a histidine-tyrosine cross-link in its heme a3/Cu B oxygen reduction center. A functional model of HCO was obtained through the genetic incorporation of the unnatural amino acid imiTyr, which mimics the Tyr-His cross-link, and of the Cu B site into myoglobin (see picture). Like HCO, this small soluble protein exhibits selective O 2-reduction activity while generating little reactive oxygen species.

AB - Top model: Heme-copper oxidase (HCO) contains a histidine-tyrosine cross-link in its heme a3/Cu B oxygen reduction center. A functional model of HCO was obtained through the genetic incorporation of the unnatural amino acid imiTyr, which mimics the Tyr-His cross-link, and of the Cu B site into myoglobin (see picture). Like HCO, this small soluble protein exhibits selective O 2-reduction activity while generating little reactive oxygen species.

KW - enzyme catalysis

KW - enzyme models

KW - oxygen reduction

KW - post-translational modifications

KW - protein design

UR - https://www.scopus.com/pages/publications/84860204636

U2 - 10.1002/anie.201108756

DO - 10.1002/anie.201108756

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SN - 1433-7851

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SP - 4312

EP - 4316

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 18

ER -

Significant increase of oxidase activity through the genetic incorporation of a tyrosine-histidine cross-link in a myoglobin model of heme-copper oxidase

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