Protease-resistant core form of Bacillus thuringiensis Cry1ie: Monomeric and oligomeric forms in solution

Shuyuan Guo; Yancai Zhang; Fuping Song; Jie Zhang; Dafang Huang

doi:10.1007/s10529-009-0078-2

Protease-resistant core form of Bacillus thuringiensis Cry1ie: Monomeric and oligomeric forms in solution

Shuyuan Guo
, Yancai Zhang
, Fuping Song
, Jie Zhang^*
, Dafang Huang

^*此作品的通讯作者

生命学院

Beijing Institute of Technology
Chinese Academy of Agricultural Sciences

科研成果: 期刊稿件 › 文章 › 同行评审

14 引用（Scopus）

摘要

Cry1Ie is encoded by cry1Ie, a gene that is silent in Bacillus thuringiensis strains but can be over-expressed in Escherichia coli. A protease-resistant core form of Cry1Ie with a molecular weight of approx. 55 kDa was found among the products of trypsin digestion. The oligomer and monomer of the protease-resistant core form were purified separately. The oligomer fraction contained a small quantity of dimer and a large amount of aggregates larger than tetramers. The 50% lethal concentrations (LC₅₀) of the full-length Cry1Ie and both the monomer and oligomer fractions of the protease-resistant core form against Plutella xylostella were 14, 21 and 1400 μg/ml, respectively.

源语言	英语
页（从-至）	1769-1774
页数	6
期刊	Biotechnology Letters
卷	31
期	11
DOI	https://doi.org/10.1007/s10529-009-0078-2
出版状态	已出版 - 10月 2009

访问文件

10.1007/s10529-009-0078-2

其它文件与链接

链接到 Scopus 的出版物

引用此

@article{396817bbe1604a048f6130e1500a2d7f,

title = "Protease-resistant core form of Bacillus thuringiensis Cry1ie: Monomeric and oligomeric forms in solution",

abstract = "Cry1Ie is encoded by cry1Ie, a gene that is silent in Bacillus thuringiensis strains but can be over-expressed in Escherichia coli. A protease-resistant core form of Cry1Ie with a molecular weight of approx. 55 kDa was found among the products of trypsin digestion. The oligomer and monomer of the protease-resistant core form were purified separately. The oligomer fraction contained a small quantity of dimer and a large amount of aggregates larger than tetramers. The 50\% lethal concentrations (LC50) of the full-length Cry1Ie and both the monomer and oligomer fractions of the protease-resistant core form against Plutella xylostella were 14, 21 and 1400 μg/ml, respectively.",

keywords = "Bacillus thuringiensis, Cry1Ie protein, Oligomer, Protease-resistant core form",

author = "Shuyuan Guo and Yancai Zhang and Fuping Song and Jie Zhang and Dafang Huang",

year = "2009",

month = oct,

doi = "10.1007/s10529-009-0078-2",

language = "English",

volume = "31",

pages = "1769--1774",

journal = "Biotechnology Letters",

issn = "0141-5492",

publisher = "Springer Science and Business Media B.V.",

number = "11",

}

TY - JOUR

T1 - Protease-resistant core form of Bacillus thuringiensis Cry1ie

T2 - Monomeric and oligomeric forms in solution

AU - Guo, Shuyuan

AU - Zhang, Yancai

AU - Song, Fuping

AU - Zhang, Jie

AU - Huang, Dafang

PY - 2009/10

Y1 - 2009/10

N2 - Cry1Ie is encoded by cry1Ie, a gene that is silent in Bacillus thuringiensis strains but can be over-expressed in Escherichia coli. A protease-resistant core form of Cry1Ie with a molecular weight of approx. 55 kDa was found among the products of trypsin digestion. The oligomer and monomer of the protease-resistant core form were purified separately. The oligomer fraction contained a small quantity of dimer and a large amount of aggregates larger than tetramers. The 50% lethal concentrations (LC50) of the full-length Cry1Ie and both the monomer and oligomer fractions of the protease-resistant core form against Plutella xylostella were 14, 21 and 1400 μg/ml, respectively.

AB - Cry1Ie is encoded by cry1Ie, a gene that is silent in Bacillus thuringiensis strains but can be over-expressed in Escherichia coli. A protease-resistant core form of Cry1Ie with a molecular weight of approx. 55 kDa was found among the products of trypsin digestion. The oligomer and monomer of the protease-resistant core form were purified separately. The oligomer fraction contained a small quantity of dimer and a large amount of aggregates larger than tetramers. The 50% lethal concentrations (LC50) of the full-length Cry1Ie and both the monomer and oligomer fractions of the protease-resistant core form against Plutella xylostella were 14, 21 and 1400 μg/ml, respectively.

KW - Bacillus thuringiensis

KW - Cry1Ie protein

KW - Oligomer

KW - Protease-resistant core form

UR - https://www.scopus.com/pages/publications/70350300627

U2 - 10.1007/s10529-009-0078-2

DO - 10.1007/s10529-009-0078-2

M3 - Article

C2 - 19590825

AN - SCOPUS:70350300627

SN - 0141-5492

VL - 31

SP - 1769

EP - 1774

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 11

ER -

Protease-resistant core form of Bacillus thuringiensis Cry1ie: Monomeric and oligomeric forms in solution

摘要

访问文件

其它文件与链接

指纹

引用此