Preparation and evaluation of 2, 6-di-O-pentyl-β-cyclodextrin bonded silica stationary phase for high performance liquid chromatography

In order to improve the chiral separation capability of the conventional β-cyclodex-trin bonded-silica gel stationary phase; 2, 6-di-O-pentyl- β-cyclodextrin bonded stationary phase (PCDS) was prepared via a long spacer. The resulted bonded-silica stationary phase was characterized by three methods, namely Fourier transform infrared, Molisch color reaction, X-ray optical electrical energy spectrogram. The chromatographic performances of PCDS were investigated by using liquid chromatography with toluene, dimethyl phthalate, and phenanthrene as solutes, and their retention mechanism was investigated and discussed. The results show that the introduction of pentyl to β-cyclodextrin leads to enhancement of the retention of the solutes. The chiral separation capability of the new bonded-silica stationary phase was evaluated by using liquid chromatography with some chiral drugs. Some of the enantiomers such as chlor-phenamine maleate and bupropion hydrochloride were separated by heptakis (2, 6-di-O-pentyl)-β-cyclodextrin bonded silica stationary.