Preparation and characterization of temperature-responsive chromatographic column containing poly(n-isopropylacrylamide) and poly([2-(methacryloyloxy)- ethyl]trimetylammonium chloride)

This article described the preparation of temperature-responsive chromatographic column containing poly(N-isopropylacrylamide) and poly quaternary ammonium salt. Poly (N-isopropylacrylamide) and poly ([2-(methacryloyloxy)ethyl]trimetylammonium chloride) were synthesized and grafted to amino modified silica gel. The temperature responsive polymer grafted silica gel was characterized using Fourier transform infrared spectroscopy, thermoweight loss, and elemental analysis. The temperature responsive chromatographic column was used to analyze lactic acid and creatine phosphate disodium salt by controlling of the column temperature from 10 to 50°C. The mixture of lactic acid and creatine phosphate disodium salt was baseline separated at pH 6.93.