Preparation and characterization of temperature-responsive capillary electrochromatographic column using poly(N-isopropylacrylamide)

Poly(N-isopropylacrylamide) is a temperature-responsive polymer, which is hydrophilic at low temperature but hydrophobic at high temperature. Using this characteristic, the polymer was bonded to the inner surface of fused-silica capillary to prepare a temperature-responsive column to study the separation behavior in electrochromatography.