Preparation and application of the chiral stationary phase of new CBZ-phenylalanine-β-cyclodextrin bonded silica for high performance liquid chromatography

CBZ-phenylalanine was reacted with 6-OH of β-cyclodextrin, then the derivative was bonded to silica to produce a new chiral stationary phase (CSP) for high performance liquid chromatography (HPLC). The derivative of β-cyclodextrin and the CSP were characterized by Molisch color reaction, IR and XPS. Chromatographic performance of the bonded silica CSP was evaluated using benzene and naphthalene as testing materials. The m-, o-, p-isomers of nitrophenol and aminophenol were separated well on the column of the CSP. Enantiomers of clenbuterol hydrochloride and 5, 6-O-isopropyl-3-thio-hexanoic acid-1, 4-lactone were investigated and tolerably separated on the column.