Local cell stiffness measurement using probes deformation

Masaru Takeuchi; Mohd Ridzuan Bin Ahmad; Masahiro Nakajima; Toshio Fukuda; Hiroki Umeshima; Mineko Kengaku; Yasuhisa Hasegawa; Qiang Huang

doi:10.1109/NANO.2014.6968035

Local cell stiffness measurement using probes deformation

Masaru Takeuchi^*
, Mohd Ridzuan Bin Ahmad
, Masahiro Nakajima
, Toshio Fukuda
, Hiroki Umeshima
, Mineko Kengaku
, Yasuhisa Hasegawa
, Qiang Huang

^*此作品的通讯作者

机电学院

Nagoya University
Universiti Teknologi Malaysia
Meijo University
Kyoto University

科研成果: 书/报告/会议事项章节 › 会议稿件 › 同行评审

3 引用（Scopus）

摘要

In this paper, we propose and demonstrate a new method for local cell stiffness measurement using multiple probes system. Thin glass pipettes were employed to measure the stiffness of cells on a general culture dish. The thin micropipettes were prepared and their spring constant was measured using the Atomic Force Microscopy (AFM) cantilever before stiffness measurement. The deformation of the pipette tips and the cell were observed under an optical microscope. The cell stiffness was calculated from the deformations based on the Hooke's low. The measurement results show that the myoblast C2C12 cell on a glass substrate has the stiffness around 0.1 N/m. The experimental results indicate that the proposed method can conduct the time-lapse cell stiffness measurement at multiple points simultaneously.

源语言	英语
主期刊名	Proceedings of the IEEE Conference on Nanotechnology
出版商	Institute of Electrical and Electronics Engineers Inc.
页	168-171
页数	4
ISBN（电子版）	9781479956227
DOI	https://doi.org/10.1109/NANO.2014.6968035
出版状态	已出版 - 26 11月 2014
活动	2014 14th IEEE International Conference on Nanotechnology, IEEE-NANO 2014 - Toronto, 加拿大期限: 18 8月 2014 → 21 8月 2014

丛书

姓名	Proceedings of the IEEE Conference on Nanotechnology
ISSN（电子版）	1944-9399

会议

会议	2014 14th IEEE International Conference on Nanotechnology, IEEE-NANO 2014
国家/地区	加拿大
市	Toronto
时期	18/08/14 → 21/08/14

访问文件

10.1109/NANO.2014.6968035

其它文件与链接

链接到 Scopus 的出版物

引用此

Takeuchi, M., Bin Ahmad, M. R., Nakajima, M., Fukuda, T., Umeshima, H., Kengaku, M., Hasegawa, Y., & Huang, Q. (2014). Local cell stiffness measurement using probes deformation. 在 Proceedings of the IEEE Conference on Nanotechnology (页码 168-171). 期刊论文 6968035 (Proceedings of the IEEE Conference on Nanotechnology). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NANO.2014.6968035

@inproceedings{a24b49b21a4840779d7d7b6781473f0a,

title = "Local cell stiffness measurement using probes deformation",

abstract = "In this paper, we propose and demonstrate a new method for local cell stiffness measurement using multiple probes system. Thin glass pipettes were employed to measure the stiffness of cells on a general culture dish. The thin micropipettes were prepared and their spring constant was measured using the Atomic Force Microscopy (AFM) cantilever before stiffness measurement. The deformation of the pipette tips and the cell were observed under an optical microscope. The cell stiffness was calculated from the deformations based on the Hooke's low. The measurement results show that the myoblast C2C12 cell on a glass substrate has the stiffness around 0.1 N/m. The experimental results indicate that the proposed method can conduct the time-lapse cell stiffness measurement at multiple points simultaneously.",

author = "Masaru Takeuchi and \{Bin Ahmad\}, \{Mohd Ridzuan\} and Masahiro Nakajima and Toshio Fukuda and Hiroki Umeshima and Mineko Kengaku and Yasuhisa Hasegawa and Qiang Huang",

note = "Publisher Copyright: {\textcopyright} 2014 IEEE.; 2014 14th IEEE International Conference on Nanotechnology, IEEE-NANO 2014 ; Conference date: 18-08-2014 Through 21-08-2014",

year = "2014",

month = nov,

day = "26",

doi = "10.1109/NANO.2014.6968035",

language = "English",

series = "Proceedings of the IEEE Conference on Nanotechnology",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

pages = "168--171",

booktitle = "Proceedings of the IEEE Conference on Nanotechnology",

address = "United States",

}

Takeuchi, M, Bin Ahmad, MR, Nakajima, M, Fukuda, T, Umeshima, H, Kengaku, M, Hasegawa, Y & Huang, Q 2014, Local cell stiffness measurement using probes deformation. 在 Proceedings of the IEEE Conference on Nanotechnology., 6968035, Proceedings of the IEEE Conference on Nanotechnology, Institute of Electrical and Electronics Engineers Inc., 页码 168-171, 2014 14th IEEE International Conference on Nanotechnology, IEEE-NANO 2014, Toronto, 加拿大, 18/08/14. https://doi.org/10.1109/NANO.2014.6968035

Local cell stiffness measurement using probes deformation. / Takeuchi, Masaru; Bin Ahmad, Mohd Ridzuan; Nakajima, Masahiro 等.
Proceedings of the IEEE Conference on Nanotechnology. Institute of Electrical and Electronics Engineers Inc., 2014. 页码 168-171 6968035 (Proceedings of the IEEE Conference on Nanotechnology).

科研成果: 书/报告/会议事项章节 › 会议稿件 › 同行评审

TY - GEN

T1 - Local cell stiffness measurement using probes deformation

AU - Takeuchi, Masaru

AU - Bin Ahmad, Mohd Ridzuan

AU - Nakajima, Masahiro

AU - Fukuda, Toshio

AU - Umeshima, Hiroki

AU - Kengaku, Mineko

AU - Hasegawa, Yasuhisa

AU - Huang, Qiang

PY - 2014/11/26

Y1 - 2014/11/26

N2 - In this paper, we propose and demonstrate a new method for local cell stiffness measurement using multiple probes system. Thin glass pipettes were employed to measure the stiffness of cells on a general culture dish. The thin micropipettes were prepared and their spring constant was measured using the Atomic Force Microscopy (AFM) cantilever before stiffness measurement. The deformation of the pipette tips and the cell were observed under an optical microscope. The cell stiffness was calculated from the deformations based on the Hooke's low. The measurement results show that the myoblast C2C12 cell on a glass substrate has the stiffness around 0.1 N/m. The experimental results indicate that the proposed method can conduct the time-lapse cell stiffness measurement at multiple points simultaneously.

AB - In this paper, we propose and demonstrate a new method for local cell stiffness measurement using multiple probes system. Thin glass pipettes were employed to measure the stiffness of cells on a general culture dish. The thin micropipettes were prepared and their spring constant was measured using the Atomic Force Microscopy (AFM) cantilever before stiffness measurement. The deformation of the pipette tips and the cell were observed under an optical microscope. The cell stiffness was calculated from the deformations based on the Hooke's low. The measurement results show that the myoblast C2C12 cell on a glass substrate has the stiffness around 0.1 N/m. The experimental results indicate that the proposed method can conduct the time-lapse cell stiffness measurement at multiple points simultaneously.

UR - https://www.scopus.com/pages/publications/84919472775

U2 - 10.1109/NANO.2014.6968035

DO - 10.1109/NANO.2014.6968035

M3 - Conference contribution

AN - SCOPUS:84919472775

T3 - Proceedings of the IEEE Conference on Nanotechnology

SP - 168

EP - 171

BT - Proceedings of the IEEE Conference on Nanotechnology

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 2014 14th IEEE International Conference on Nanotechnology, IEEE-NANO 2014

Y2 - 18 August 2014 through 21 August 2014

ER -

Local cell stiffness measurement using probes deformation

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