Lectin chip for individally isolating of pancereatic circulating tumor cells

To achieve analysis of CTCs at the single-cell level, we have applied the meniscus-induced microfluidic device, rare cell sorter. However, size-based isolation causes missing of smaller cells. In this paper, we proposed an open-channel chip that immobilized a specific lectin to assist size-based cell isolation. Immobilized lectins was used to bind a glycoprotein of pancreatic cancer cell. In this work, We demonstrated that the lectin chip with rBC2LCN provided high isolation rate and predominantly assist size-based isolation.