Investigation about 3D microscopical image restoration and PSF

The deconvolution for 3D image of wide-field microscope need to process very large data size, and it spend a lot of time. 3D PSF (three dimensional point spread function), out-of-focus images, optical section images and restored images deconvoluted by different 3D PSF were estimated and analyzed. It was proved further that the disturbance of out-of-focus light to in-focus images was from out-of-focus images of both sides. It was proposed that 3D microscopy deconvolution could get more perfect effect of image restoration and the runtime could be spend less through selecting appropriate size of 3D PSF. And a new standard for estimating the definition or fuzzy degree of image was proposed, and it was applied to estimation of out-of-focus images, optical section images and restored images.