Generation of an ASS1 heterozygous knockout human embryonic stem cell line, WAe001-A-13, using CRISPR/Cas9

Fang Yuan; Dongsheng Guo; Yanli Liu; Yingying Xu; Ge Gao; Yuhang Wu; Fan Yang; Xinrong Ke; Keyu Lai; Hongcheng Wei; Yin Xiong Li

doi:10.1016/j.scr.2017.11.007

Generation of an ASS1 heterozygous knockout human embryonic stem cell line, WAe001-A-13, using CRISPR/Cas9

Fang Yuan
, Dongsheng Guo
, Yanli Liu
, Yingying Xu
, Ge Gao
, Yuhang Wu
, Fan Yang
, Xinrong Ke
, Keyu Lai
, Hongcheng Wei^*
, Yin Xiong Li

^*此作品的通讯作者

CAS - Guangzhou Institute of Biomedicine and Health
University of Science and Technology of China
Chinese Academy of Sciences
University of Chinese Academy of Sciences
The First Affiliated Hospital of Jinan University

科研成果: 期刊稿件 › 文章 › 同行评审

2 引用（Scopus）

摘要

The ASS1 gene encodes argininosuccinate synthetase-1, a cytosolic enzyme with a critical role in the urea cycle. Mutations are found in all ASS1 exons and cause the autosomal recessive disorder citrullinemia. Using CRISPR/Cas9-editing, we established the WAe001-A-13 cell line, which was heterozygous for an ASS1 mutation, from the human embryonic stem cell line H1. The WAe001-A-13 cell line maintained the pluripotent phenotype, the ability to differentiate into all three germ layers and a normal karyotype.

源语言	英语
页（从-至）	67-71
页数	5
期刊	Stem Cell Research
卷	26
DOI	https://doi.org/10.1016/j.scr.2017.11.007
出版状态	已出版 - 1月 2018
已对外发布	是

访问文件

10.1016/j.scr.2017.11.007

其它文件与链接

链接到 Scopus 的出版物

引用此

@article{b02754535363444f95ddf6a8cb2cce61,

title = "Generation of an ASS1 heterozygous knockout human embryonic stem cell line, WAe001-A-13, using CRISPR/Cas9",

abstract = "The ASS1 gene encodes argininosuccinate synthetase-1, a cytosolic enzyme with a critical role in the urea cycle. Mutations are found in all ASS1 exons and cause the autosomal recessive disorder citrullinemia. Using CRISPR/Cas9-editing, we established the WAe001-A-13 cell line, which was heterozygous for an ASS1 mutation, from the human embryonic stem cell line H1. The WAe001-A-13 cell line maintained the pluripotent phenotype, the ability to differentiate into all three germ layers and a normal karyotype.",

author = "Fang Yuan and Dongsheng Guo and Yanli Liu and Yingying Xu and Ge Gao and Yuhang Wu and Fan Yang and Xinrong Ke and Keyu Lai and Hongcheng Wei and Li, \{Yin Xiong\}",

note = "Publisher Copyright: {\textcopyright} 2017",

year = "2018",

month = jan,

doi = "10.1016/j.scr.2017.11.007",

language = "English",

volume = "26",

pages = "67--71",

journal = "Stem Cell Research",

issn = "1873-5061",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Generation of an ASS1 heterozygous knockout human embryonic stem cell line, WAe001-A-13, using CRISPR/Cas9

AU - Yuan, Fang

AU - Guo, Dongsheng

AU - Liu, Yanli

AU - Xu, Yingying

AU - Gao, Ge

AU - Wu, Yuhang

AU - Yang, Fan

AU - Ke, Xinrong

AU - Lai, Keyu

AU - Wei, Hongcheng

AU - Li, Yin Xiong

PY - 2018/1

Y1 - 2018/1

N2 - The ASS1 gene encodes argininosuccinate synthetase-1, a cytosolic enzyme with a critical role in the urea cycle. Mutations are found in all ASS1 exons and cause the autosomal recessive disorder citrullinemia. Using CRISPR/Cas9-editing, we established the WAe001-A-13 cell line, which was heterozygous for an ASS1 mutation, from the human embryonic stem cell line H1. The WAe001-A-13 cell line maintained the pluripotent phenotype, the ability to differentiate into all three germ layers and a normal karyotype.

AB - The ASS1 gene encodes argininosuccinate synthetase-1, a cytosolic enzyme with a critical role in the urea cycle. Mutations are found in all ASS1 exons and cause the autosomal recessive disorder citrullinemia. Using CRISPR/Cas9-editing, we established the WAe001-A-13 cell line, which was heterozygous for an ASS1 mutation, from the human embryonic stem cell line H1. The WAe001-A-13 cell line maintained the pluripotent phenotype, the ability to differentiate into all three germ layers and a normal karyotype.

UR - https://www.scopus.com/pages/publications/85037986258

U2 - 10.1016/j.scr.2017.11.007

DO - 10.1016/j.scr.2017.11.007

M3 - Article

C2 - 29247816

AN - SCOPUS:85037986258

SN - 1873-5061

VL - 26

SP - 67

EP - 71

JO - Stem Cell Research

JF - Stem Cell Research

ER -

Generation of an ASS1 heterozygous knockout human embryonic stem cell line, WAe001-A-13, using CRISPR/Cas9

摘要

访问文件

其它文件与链接

学术指纹

引用此