Generation of a KCNJ11 homozygous knockout human embryonic stem cell line WAe001-A-12 using CRISPR/Cas9

Fang Yuan; Dongsheng Guo; Ge Gao; Yanli Liu; Yingying Xu; Yuhang Wu; Fan Yang; Xinrong Ke; Keyu Lai; Liangqing Hong; Yin xiong Li

doi:10.1016/j.scr.2017.08.016

Generation of a KCNJ11 homozygous knockout human embryonic stem cell line WAe001-A-12 using CRISPR/Cas9

Fang Yuan
, Dongsheng Guo
, Ge Gao
, Yanli Liu
, Yingying Xu
, Yuhang Wu
, Fan Yang
, Xinrong Ke
, Keyu Lai
, Liangqing Hong^*
, Yin xiong Li

^*此作品的通讯作者

University of Science and Technology of China
CAS - Guangzhou Institute of Biomedicine and Health
University of Chinese Academy of Sciences
The Third Affiliated Hospital of Sun Yat-sen University

科研成果: 期刊稿件 › 文章 › 同行评审

3 引用（Scopus）

摘要

The ATP-sensitive potassium channel is an octameric complex, and one of its subunits, namely Kir6.2, is encoded by the KCNJ11 gene. Mutations in KCNJ11 result in hyperinsulinism or diabetes mellitus, associated with abnormal insulin secretion. Here, using CRISPR/Cas9 editing, we established a homozygous mutant KCNJ11 cell line, WAe001-A-12, which was generated by a 62-bp deletion in the coding sequence of the human embryonic stem cell line H1. It was confirmed that this deletion in the KCNJ11 gene did not affect the protein expression levels of key pluripotent factors. Additionally, normal karyotype and differentiation potency were observed for the cell line.

源语言	英语
页（从-至）	89-93
页数	5
期刊	Stem Cell Research
卷	24
DOI	https://doi.org/10.1016/j.scr.2017.08.016
出版状态	已出版 - 10月 2017
已对外发布	是

联合国可持续发展目标

此成果有助于实现下列可持续发展目标：

可持续发展目标 3 良好健康与福祉

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10.1016/j.scr.2017.08.016

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title = "Generation of a KCNJ11 homozygous knockout human embryonic stem cell line WAe001-A-12 using CRISPR/Cas9",

abstract = "The ATP-sensitive potassium channel is an octameric complex, and one of its subunits, namely Kir6.2, is encoded by the KCNJ11 gene. Mutations in KCNJ11 result in hyperinsulinism or diabetes mellitus, associated with abnormal insulin secretion. Here, using CRISPR/Cas9 editing, we established a homozygous mutant KCNJ11 cell line, WAe001-A-12, which was generated by a 62-bp deletion in the coding sequence of the human embryonic stem cell line H1. It was confirmed that this deletion in the KCNJ11 gene did not affect the protein expression levels of key pluripotent factors. Additionally, normal karyotype and differentiation potency were observed for the cell line.",

author = "Fang Yuan and Dongsheng Guo and Ge Gao and Yanli Liu and Yingying Xu and Yuhang Wu and Fan Yang and Xinrong Ke and Keyu Lai and Liangqing Hong and Li, \{Yin xiong\}",

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doi = "10.1016/j.scr.2017.08.016",

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T1 - Generation of a KCNJ11 homozygous knockout human embryonic stem cell line WAe001-A-12 using CRISPR/Cas9

AU - Yuan, Fang

AU - Guo, Dongsheng

AU - Gao, Ge

AU - Liu, Yanli

AU - Xu, Yingying

AU - Wu, Yuhang

AU - Yang, Fan

AU - Ke, Xinrong

AU - Lai, Keyu

AU - Hong, Liangqing

AU - Li, Yin xiong

PY - 2017/10

Y1 - 2017/10

N2 - The ATP-sensitive potassium channel is an octameric complex, and one of its subunits, namely Kir6.2, is encoded by the KCNJ11 gene. Mutations in KCNJ11 result in hyperinsulinism or diabetes mellitus, associated with abnormal insulin secretion. Here, using CRISPR/Cas9 editing, we established a homozygous mutant KCNJ11 cell line, WAe001-A-12, which was generated by a 62-bp deletion in the coding sequence of the human embryonic stem cell line H1. It was confirmed that this deletion in the KCNJ11 gene did not affect the protein expression levels of key pluripotent factors. Additionally, normal karyotype and differentiation potency were observed for the cell line.

AB - The ATP-sensitive potassium channel is an octameric complex, and one of its subunits, namely Kir6.2, is encoded by the KCNJ11 gene. Mutations in KCNJ11 result in hyperinsulinism or diabetes mellitus, associated with abnormal insulin secretion. Here, using CRISPR/Cas9 editing, we established a homozygous mutant KCNJ11 cell line, WAe001-A-12, which was generated by a 62-bp deletion in the coding sequence of the human embryonic stem cell line H1. It was confirmed that this deletion in the KCNJ11 gene did not affect the protein expression levels of key pluripotent factors. Additionally, normal karyotype and differentiation potency were observed for the cell line.

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DO - 10.1016/j.scr.2017.08.016

M3 - Article

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AN - SCOPUS:85028074922

SN - 1873-5061

VL - 24

SP - 89

EP - 93

JO - Stem Cell Research

JF - Stem Cell Research

ER -

Generation of a KCNJ11 homozygous knockout human embryonic stem cell line WAe001-A-12 using CRISPR/Cas9

摘要

联合国可持续发展目标

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