TY - JOUR
T1 - DNA-assembled nanoarchitectures with multiple components in regulated and coordinated motion
AU - Zhan, Pengfei
AU - Urban, Maximilian J.
AU - Both, Steffen
AU - Duan, Xiaoyang
AU - Kuzyk, Anton
AU - Weiss, Thomas
AU - Liu, Na
N1 - Publisher Copyright:
Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).
PY - 2019/11/29
Y1 - 2019/11/29
N2 - Coordinating functional parts to operate in concert is essential for machinery. In gear trains, meshed gears are compactly interlocked, working together to impose rotation or translation. In photosynthetic systems, a variety of biological entities in the thylakoid membrane interact with each other, converting light energy into chemical energy. However, coordinating individual parts to carry out regulated and coordinated motion within an artificial nanoarchitecture poses challenges, owing to the requisite control on the nanoscale. Here, we demonstrate DNA-directed nanosystems, which comprise hierarchically-assembled DNA origami filaments, fluorophores, and gold nanocrystals. These individual building blocks can execute independent, synchronous, or joint motion upon external inputs. These are optically monitored in situ using fluorescence spectroscopy, taking advantage of the sensitive distance-dependent interactions between the gold nanocrystals and fluorophores positioned on the DNA origami. Our work leverages the complexity of DNA-based artificial nanosystems with tailored dynamic functionality, representing a viable route towards technomimetic nanomachinery.
AB - Coordinating functional parts to operate in concert is essential for machinery. In gear trains, meshed gears are compactly interlocked, working together to impose rotation or translation. In photosynthetic systems, a variety of biological entities in the thylakoid membrane interact with each other, converting light energy into chemical energy. However, coordinating individual parts to carry out regulated and coordinated motion within an artificial nanoarchitecture poses challenges, owing to the requisite control on the nanoscale. Here, we demonstrate DNA-directed nanosystems, which comprise hierarchically-assembled DNA origami filaments, fluorophores, and gold nanocrystals. These individual building blocks can execute independent, synchronous, or joint motion upon external inputs. These are optically monitored in situ using fluorescence spectroscopy, taking advantage of the sensitive distance-dependent interactions between the gold nanocrystals and fluorophores positioned on the DNA origami. Our work leverages the complexity of DNA-based artificial nanosystems with tailored dynamic functionality, representing a viable route towards technomimetic nanomachinery.
UR - https://www.scopus.com/pages/publications/85076307379
U2 - 10.1126/sciadv.aax6023
DO - 10.1126/sciadv.aax6023
M3 - Article
C2 - 31819901
AN - SCOPUS:85076307379
SN - 2375-2548
VL - 5
JO - Science advances
JF - Science advances
IS - 11
M1 - eaax6023
ER -