TY - JOUR
T1 - Development and performance validation of an affordable and portable high-resolution darkfield polarization-sensitive multispectral imaging microscope for the assessment of radiation dermatitis and fibrosis
AU - Hao, Shicheng
AU - Guo, Sisi
AU - Chen, Shuyu
AU - Wang, Hao
AU - Chen, Qian
AU - Zhou, Xudong
AU - Liu, Lihui
AU - Zhang, Aijun
AU - Sun, Hui
AU - Zhang, Ruoyu
AU - Wang, Jianfeng
N1 - Publisher Copyright:
© 2024 Optica Publishing Group.
PY - 2025/1
Y1 - 2025/1
N2 - Radiation therapy (RT) is widely used for cancer treatment but is found with side effects of radiation dermatitis and fibrosis thereby calling for timely assessment. Nevertheless, current clinical assessment methods are found to be subjective, prone to bias, and accompanied by variability. There is, therefore, an unmet clinical need to explore a new assessment technique, ideally portable and affordable, making it accessible to less developed regions too. We developed an affordable (16764 CNY) and portable high-resolution ((3.91 μm) darkfield polarizationsensitive multispectral imaging (PS-MSI) microscope. The implementation of the Monte Carlo simulation on the PS multi spectra allows the quantitative analysis of physiological parameters (i.e., blood volume fraction (BVF) and oxygen saturation of hemoglobin) at different skin layers for the dermatitis assessment. Further derivation of the degree of linear polarization (DOLP) reflects randomly distributed collagen fibers associated with fibrosis for the fibrosis assessment. PS-MSI microscope developed revealed a significant decrease (p < 0.001, analysis of variance, ANOVA) in the DOLP associated with fibrosis like scar tissue, and significant (p < 0.001, ANOVA) increases in BVF and oxygen saturation of hemoglobin accompanying artificially induced dermatitis. One-dimensional convolutional neural network implemented on the DOLP and multiple spectra achieved accuracies of 96% and 92.2%, respectively, for the classification of the artificially induced skin dermatitis and fibrosis like scar, demonstrating the potential of the affordable PS-MSI microscope developed for objective, unbiased and consistent assessment of radiation dermatitis and fibrosis in the clinics.
AB - Radiation therapy (RT) is widely used for cancer treatment but is found with side effects of radiation dermatitis and fibrosis thereby calling for timely assessment. Nevertheless, current clinical assessment methods are found to be subjective, prone to bias, and accompanied by variability. There is, therefore, an unmet clinical need to explore a new assessment technique, ideally portable and affordable, making it accessible to less developed regions too. We developed an affordable (16764 CNY) and portable high-resolution ((3.91 μm) darkfield polarizationsensitive multispectral imaging (PS-MSI) microscope. The implementation of the Monte Carlo simulation on the PS multi spectra allows the quantitative analysis of physiological parameters (i.e., blood volume fraction (BVF) and oxygen saturation of hemoglobin) at different skin layers for the dermatitis assessment. Further derivation of the degree of linear polarization (DOLP) reflects randomly distributed collagen fibers associated with fibrosis for the fibrosis assessment. PS-MSI microscope developed revealed a significant decrease (p < 0.001, analysis of variance, ANOVA) in the DOLP associated with fibrosis like scar tissue, and significant (p < 0.001, ANOVA) increases in BVF and oxygen saturation of hemoglobin accompanying artificially induced dermatitis. One-dimensional convolutional neural network implemented on the DOLP and multiple spectra achieved accuracies of 96% and 92.2%, respectively, for the classification of the artificially induced skin dermatitis and fibrosis like scar, demonstrating the potential of the affordable PS-MSI microscope developed for objective, unbiased and consistent assessment of radiation dermatitis and fibrosis in the clinics.
UR - http://www.scopus.com/inward/record.url?scp=85213818572&partnerID=8YFLogxK
U2 - 10.1364/BOE.546226
DO - 10.1364/BOE.546226
M3 - Article
AN - SCOPUS:85213818572
SN - 2156-7085
VL - 16
SP - 320
EP - 333
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 1
ER -