TY - GEN
T1 - Determination of S-catechol-O-methyltransferase activity in rat tissues using high-performance liquid chromatography with electrochemical detection
AU - Lina, Yang
AU - Yujuan, Li
AU - Shengyuan, Xiao
AU - Yulin, Deng
PY - 2009
Y1 - 2009
N2 - A high-performance liquid chromatography with electrochemical detection (HPLC-ECD) method was developed to determine the activity of S-catechol-O-methyltransferase (COMT) in rat tissues including the liver, kidney, lung ,spleen and brain. The 3,4-dihydroxybenzoic acid was used as the substrate for SCOMT in rat tissues. After incubated with different tissues, the product 4-hydroxy-3-methoxybenzoic acid was formed from the substrate and separated to measure the COMT activity. The analysis was performed on a Agilent C8 column (5μm, 150x4.6mm) at 550mV potential. The mobile phase was a mixture of methanol and 0.1MNa2HPO4(pH=3.3) containing 0.13mM ethylenediamine tetraacetic acid (v/v, 14/86) with a flow rate of 1ml/min. This approach was used to study S-COMT activity in vitro from various tissues.
AB - A high-performance liquid chromatography with electrochemical detection (HPLC-ECD) method was developed to determine the activity of S-catechol-O-methyltransferase (COMT) in rat tissues including the liver, kidney, lung ,spleen and brain. The 3,4-dihydroxybenzoic acid was used as the substrate for SCOMT in rat tissues. After incubated with different tissues, the product 4-hydroxy-3-methoxybenzoic acid was formed from the substrate and separated to measure the COMT activity. The analysis was performed on a Agilent C8 column (5μm, 150x4.6mm) at 550mV potential. The mobile phase was a mixture of methanol and 0.1MNa2HPO4(pH=3.3) containing 0.13mM ethylenediamine tetraacetic acid (v/v, 14/86) with a flow rate of 1ml/min. This approach was used to study S-COMT activity in vitro from various tissues.
KW - 4-hydroxy-3-methoxybenzoic acid
KW - Catechol-O-methyltransferase
KW - HPLC-ECD
KW - Rat tissues
UR - https://www.scopus.com/pages/publications/72749092242
U2 - 10.1109/ICBBE.2009.5162625
DO - 10.1109/ICBBE.2009.5162625
M3 - Conference contribution
AN - SCOPUS:72749092242
SN - 9781424429028
T3 - 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009
BT - 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009
T2 - 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009
Y2 - 11 June 2009 through 13 June 2009
ER -