Crawling and adhesion behavior of Halamphora sp. based on different parts of Folium Sennae-like film: Evaluation of analytical methods for anti-diatom experimental results

Anti-diatom testing is a basic method to evaluate the anti-fouling performance of coatings. Many existing results of anti-diatom performances are evaluated based on their attachment number or coverage area, ignoring the influence of the crawling and adhesion behavior of diatoms on the analysis results. Here, a Folium Sennae-like film with multiple structural units was prepared by considering the influence of diatom attachment behaviors on the analysis results. The anti-diatom performances of different parts (divided and called four parts: edge, surface, cross striation, and vertical pattern) on the Folium Sennae-like film were evaluated using the counting and area methods. Obviously, the anti-diatom performance of the Folium Sennae-like film was superior to that of epoxy resin without structure. Under equal areas, the average numbers of diatoms on the cross striation and the vertical pattern were similar to the surface. It was found that the attachment behavior of Halamphora sp. is affected by microstructure units, rather than the combined structure of which the scale is much larger than that of diatoms. Meanwhile, the average attachment area for the unit number of diatoms was calculated. The diatom attachment area without microstructure, surface, cross striation, or vertical pattern was 81.751, 106.950, 73.904, and 84.376 μm², respectively. Moreover, the static and dynamic motion behaviors of Halamphora sp. were studied, and the theory for Halamphora sp. attachment was modeled in three dimensions. The variable morphology of Halamphora sp. lead to inaccurate results for diatom analyses based on the counting and area methods, which is summarized here. This study discusses the evaluation method of coatings by anti-diatom performance, further promoting the research of diatoms in the field of antifouling.