Chemical Composition Analysis and Characterization of Reference Sample of Huangqi Guizhi Wuwutang Based on UPLC-Q-TOF-MS

［Abstract］ Objective：To analyze the chemical composition of the reference sample of Huangqi Guizhi Wuwutang（lyophilized powder），and to provide quality markers for the formulation of quality standards of this formula. Method： Ultra performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry（UPLC-Q-TOF-MS）was performed on a Waters ACQUITY UPLC ™ HSS T3 column（2.1 mm× 100 mm，1.8 μm），the mobile phase was methanol（A）-0.1% formic acid aqueous solution（B）for gradient elution（0-8 min，1%-20%A；8-10 min，20%-30%A；10-12 min，30%-35%A；12-14 min，35%-40%A，14-23 min，40%-55%A，23-27 min，55%-99%A；27-28 min，99%A；28-28.5 min，99%-1%A；28.5-30 min， 1%A），the column temperature was 40 ℃，the injection volume was 2 μL，and the flow rate was 0.3 mL·min^-1. The mass spectrometry data of the reference sample of Huangqi Guizhi Wuwutang（lyophilized powder）were collected under positive and negative ion modes. The conditions of mass spectrometry were electrospray ionization（ESI），scanning range of m/z 50-1 200，and impact energy of 10-30 eV. UNIFI 1.8 and Progenesis QI 2.0 software were used to analyze and characterize the chemical constituents in reference sample of Huangqi Guizhi Wuwutang（lyophilized powder）combined with reference comparison and literature review. Result：A total of 123 chemical constituents were identified，including 33 flavonoids，26 glycosides，18 organic acids， 11 terpenoids，7 phenylpropanoids，4 gingerol，3 alkaloids，3 amino acids，2 amides and 16 other compounds. Conclusion： The established method can quickly and accurately characterize the chemical components in the reference sample of Huangqi Guizhi Wuwutang（lyophilized powder），which can provide a basis for the selection of quality evaluation indicators of this formula，and provide a reference for its preparation research.