Cell image segmentation and tracking based on VFC Snake model in vivo

Cellular morphology contains the significant healthy information of the living organisms. Morphological analysis of cells features conduces to classify normal physiology and abnormal pathology. Lymphocyte is one most important cell of the organismal immune system. For an organ transplantation recipient, the body has an immune response, transplant rejection, to the transplanted organ. Lymphocyte morphology will distort sharply. Cellular morphology analysis in membrane boundaries can provide important information for repulsion diagnosis. Clinical lymphocyte microscopic video is segmented and tracked with mathematical morphology, the VFC Snake model. The test results show that cellular morphologic distortion has close relation with transplant rejection.