Binding mechanism of meso-tetrakis (4-(N-methylpyridiumyl)) porphyrin TMPyP4 with the single-strand DNA 5'-TTAGGG-3'

Hui Juan Zhang; Xu Kun Chen; Si Ping Pang

Binding mechanism of meso-tetrakis (4-(N-methylpyridiumyl)) porphyrin TMPyP4 with the single-strand DNA 5'-TTAGGG-3'

Hui Juan Zhang^*
, Xu Kun Chen
, Si Ping Pang

^*此作品的通讯作者

材料学院

Beijing Institute of Technology

科研成果: 期刊稿件 › 文章 › 同行评审

摘要

The binding mechanism of meso-tetrakis (4-(N-methylpyridiumyl)) porphyrin (TMPyP4) and the telomeric sequence DNA 5'-TTAGGG-3' (S6) has been explored by means of circular dichroism spectrum, steady-state absorption, steady-state fluorescence and picosecond time-resolved fluorescence spectrums. DNA adopts the single-strand conformation in Tris-HCl buffer without any metal ions. The binding constant and the saturated binding number between TMPyP4 and S6 DNA were determined as 1.51 × 10⁶ (mol/L)^-1 and 1.2, respectively, according to steady-state absorption spectroscopy. Furthermore, on the findings of time-resolved fluorescence spectroscopic technique, the binding modes can be presumed.

源语言	英语
页（从-至）	1365-1368+1373
期刊	Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology
卷	30
期	11
出版状态	已出版 - 11月 2010

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title = "Binding mechanism of meso-tetrakis (4-(N-methylpyridiumyl)) porphyrin TMPyP4 with the single-strand DNA 5'-TTAGGG-3'",

abstract = "The binding mechanism of meso-tetrakis (4-(N-methylpyridiumyl)) porphyrin (TMPyP4) and the telomeric sequence DNA 5'-TTAGGG-3' (S6) has been explored by means of circular dichroism spectrum, steady-state absorption, steady-state fluorescence and picosecond time-resolved fluorescence spectrums. DNA adopts the single-strand conformation in Tris-HCl buffer without any metal ions. The binding constant and the saturated binding number between TMPyP4 and S6 DNA were determined as 1.51 × 106 (mol/L)-1 and 1.2, respectively, according to steady-state absorption spectroscopy. Furthermore, on the findings of time-resolved fluorescence spectroscopic technique, the binding modes can be presumed.",

keywords = "Binding mechanism, Single-strand DNA, Spectroscopic technique, TMPyP4",

author = "Zhang, \{Hui Juan\} and Chen, \{Xu Kun\} and Pang, \{Si Ping\}",

year = "2010",

month = nov,

language = "English",

volume = "30",

pages = "1365--1368+1373",

journal = "Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology",

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TY - JOUR

T1 - Binding mechanism of meso-tetrakis (4-(N-methylpyridiumyl)) porphyrin TMPyP4 with the single-strand DNA 5'-TTAGGG-3'

AU - Zhang, Hui Juan

AU - Chen, Xu Kun

AU - Pang, Si Ping

PY - 2010/11

Y1 - 2010/11

N2 - The binding mechanism of meso-tetrakis (4-(N-methylpyridiumyl)) porphyrin (TMPyP4) and the telomeric sequence DNA 5'-TTAGGG-3' (S6) has been explored by means of circular dichroism spectrum, steady-state absorption, steady-state fluorescence and picosecond time-resolved fluorescence spectrums. DNA adopts the single-strand conformation in Tris-HCl buffer without any metal ions. The binding constant and the saturated binding number between TMPyP4 and S6 DNA were determined as 1.51 × 106 (mol/L)-1 and 1.2, respectively, according to steady-state absorption spectroscopy. Furthermore, on the findings of time-resolved fluorescence spectroscopic technique, the binding modes can be presumed.

AB - The binding mechanism of meso-tetrakis (4-(N-methylpyridiumyl)) porphyrin (TMPyP4) and the telomeric sequence DNA 5'-TTAGGG-3' (S6) has been explored by means of circular dichroism spectrum, steady-state absorption, steady-state fluorescence and picosecond time-resolved fluorescence spectrums. DNA adopts the single-strand conformation in Tris-HCl buffer without any metal ions. The binding constant and the saturated binding number between TMPyP4 and S6 DNA were determined as 1.51 × 106 (mol/L)-1 and 1.2, respectively, according to steady-state absorption spectroscopy. Furthermore, on the findings of time-resolved fluorescence spectroscopic technique, the binding modes can be presumed.

KW - Binding mechanism

KW - Single-strand DNA

KW - Spectroscopic technique

KW - TMPyP4

UR - https://www.scopus.com/pages/publications/78650364300

M3 - Article

AN - SCOPUS:78650364300

SN - 1001-0645

VL - 30

SP - 1365-1368+1373

JO - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology

JF - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology

IS - 11

ER -

Binding mechanism of meso-tetrakis (4-(N-methylpyridiumyl)) porphyrin TMPyP4 with the single-strand DNA 5'-TTAGGG-3'

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