Bimodal polarization-sensitive imaging and diffuse reflectance spectroscopy facilitated rapid augmented reality for tissue stratification

We reported the development of a bimodal large field of view (~95 cm²) polarization-sensitive (PS) imaging and point-wise diffuse reflectance (DR) spectroscopy platform synergising the advantages of the two modalities. PS-DR spectroscopy allows simultaneous label-free biochemical and structural analysis of the investigated tissue. For real applications, the PS imaging first grossly stratified tissue into different categories, followed by high information dimension PS-DR spectroscopy ascertaining ambiguous tissue regions identified by the PS imaging. Tested on a tissue phantom consisting of porcine esophagus muscularis propria and submucosa, it has demonstrated the system developed could complete tissue interrogation within 0.6 minutes and with separation accuracy of 95.87%. Further test on tissue phantom consisting of natural and crushed bovine tendon mimicking normal and cancerous breast tissues validated the potential of system developed to effectively and rapidly investigate large tissue area, facilitating augmented tissue boundary demarcation.